INDUKSI POLISAKARIDA KRESTIN DARI EKSTRAK Coriolus versicolor TERHADAP KONSENTASI IL-23 DAN JUMLAH NEKROSIS HEPATOSIT Mus musculus YANG DIINFEKSI Klebsiella pneumoniae
| Main Author: | RENNA INTAN RUMANIKASIH, 081211431006 |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | ind |
| Terbitan: |
, 2016
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/52615/1/MPB%2056%20-16%20Rum%20i-Abstrak.pdf http://repository.unair.ac.id/52615/2/MPB%2056%20-16%20Rum%20i.pdf http://repository.unair.ac.id/52615/ http://lib.unair.ac.id |
Daftar Isi:
- This research was aimed to know the induction of polysaccharide krestin from Coriolus versicolor extract on IL-23 concentration and hepatocyte necrosis of mice infected by K. pneumoniae. Thirty adult female mice of Mus musculus strain Balb/C, age 8 – 10 weeks and weight 30 – 40 g, were sacrificed. Mice were divided into six treatment groups, that were: group K was control; group K+ was positive control; group K- was negative control; group P1 was added PSK before being exposed by K. pneumoniae; group P2 was added PSK after being exposed by K. pneumoniae; group P3 was added PSK before and after being exposed by K. pneumoniae. Every treatment had four replications. PSK was added 100 mg/kg body weight in dose while K. pneumoniae were infected in 1,5 x 108 cell bactery. IL-23 concentration was determined by Mouse ELISA Kit and hepatocyte necrosis were made to histology slide. Data of IL-23 concentration were analyzed by Brown Forsythe test and Games Howell test (α = 0,05). Data of hepatocyte necrosis concentration were analyzed by One Way ANOVA and Duncan test (α = 0,05). The result of this research showed that P3 had the highest IL-23 concentration (141,5 pg/mL) and K- had the lowest IL-23 concentration (141,5 pg/mL). IL-23 concentrations of K, K+, P1 and P2 were 77,98 pg/mL, 88,81 pg/mL, 72,24 pg/mL and 64,28 pg/mL respectively. Meanwhile, the highest average amount of hepatocyte necrosis was K- (14,56 cell/cm2) and the lowest average amount of hepatocyte necrosis was K (11,42 cell/cm2). Average amount of hepatocyte necrosis in K+, P1, P2, and P3 were 13,57 cell/cm2, 13,75 cell/cm2, 14,29 cell/cm2 and 12,97 cell/cm2 respectively. The conclusion of this research was that PSK could increase IL-23 concentration and decrease hepatocyte necrosis of mice with before and after more infected K. pneumoniae as potentialtime.