EXPRESSION OF SOYBEAN β-1,3-ENDOGLUCANASE CDNA AND EFFECT ON DISEASE TOLERANCE IN TRANSGENIC CABBAGE PLANTS
| Main Authors: | Manuhara, Yosephine Sri Wulan, Sumardi, Issirep, Sudjadi, Sismindari, Taryono, |
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| Format: | Proceeding PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Universitas Airlangga
, 2006
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/45610/3/Abstrak.pdf http://repository.unair.ac.id/45610/1/Bukti%20C16%20-%20Expression%20of%20Soybean%20b-1%2C3-endoglucanase%20cDNA.pdf http://repository.unair.ac.id/45610/2/Reviewer%20dan%20Validasi%20Bukti%20C16.pdf http://repository.unair.ac.id/45610/ |
Daftar Isi:
- Cabbage (Brassica oleracea cv. capitata L.) is one of the main and important upland vegetables in Indonesia. However, growing cabbage is facing so many problems, especially fungal disease where there is no resistance gene found. Therefore, the development of cabbage resistant to fungal disease through the use of unconventional method is utmost important. Agrobacterium-mediated transformation of soybean β-1,3-endoglucanase cDNA, a fungal disease resistance gene, into cabbage genome has been done and got seven cabbage transformant. The expression of soybean β-1,3-endoglucanase cDNA in cabbage transformant was checked by dot blot hybridization of total RNA of recombinant planlet using amplified β-1,3-endoglucanase cDNA fragment as a probe. Disease tolerant of the recombinant cabbage was analyzed by inoculating the recombinant planlets using Fusarium sp. Analysis of soybean β-1,3-endoglucanase cDNA expression demonstrated that four out of seven recombinant cabbage planlets were able to express transformed gene, and three of them showed a complete resistance to Fusarium sp infection. However one of them showed a partial resistance as demonstrated by appearance of necrotic area that was smaller than untransformed cabbage.