Produksi equine chorionic gonadotropin (eCG) frozen dry dari serum kuda bunting lokal untuk peningkatan kebuntingan sapi Madura
| Main Author: | HERRY AGOES HERMADI, 2012 |
|---|---|
| Format: | Lainnya NonPeerReviewed Book |
| Bahasa: | ind |
| Terbitan: |
UNIVERSITAS AIRLANGGA
, 2012
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/43182/1/gdlhub-gdl-res-2013-hermadiher-27492-lp.58-13-p.pdf http://repository.unair.ac.id/43182/ http://lib.unair.ac.id |
Daftar Isi:
- The aim of this research was to produce eCO from the pregnant mares sera. This study identified eCO by conformation of the glycoprotein characteristic. the pregnant mares sera were collected from 4 horses. The protein filtrated by using sephadex 0-100. The result of SDS-PAOE with protein bands ranged between 65, 55 and 28 kDa and continued of western blot and dot blot revealed immunerectivity by monoclonal antibody of eeO of the 55 kDa bands. The cocentration ofeeO 5.12501 mIU/ml by indirect elisa