PENENTUAN MUTASI EKSON 9 DARI GEN PIK3CA PADA PASIEN KANKER PAYUDARA DI SUMATERA BARAT
| Main Author: | Dio, Aldo Rano |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | ind |
| Terbitan: |
, 2017
|
| Subjects: | |
| Online Access: |
http://scholar.unand.ac.id/27014/1/Cover%20%2B%20Abstrak.pdf http://scholar.unand.ac.id/27014/2/Bab%20I.pdf http://scholar.unand.ac.id/27014/3/Bab%20V.pdf http://scholar.unand.ac.id/27014/4/Daftar%20Pustaka.pdf http://scholar.unand.ac.id/27014/5/Skripsi%20OK%20Watermark.pdf http://scholar.unand.ac.id/27014/ |
Daftar Isi:
- The mutation of PIK3CA gene altered the activation of phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) in controlling cell proliferation and induced cancer cell formation. Aim of this study is to determine exon 9 mutation of PIK3CA gene in breast cancer by using PCR method and Sanger sequencing method. Genomic DNA was isolated from ten breast carcinomas. Exon 9 of PIK3CA gene was amplified by using specific primer. Furthermore, the purified PCR products were sequenced. The exon 9 of PIK3CA gene sequences was analyzed by Geneious software. The isolated genomics DNA with concentration between 80-140 ng/μL were used to amplify 321 bp PCR products harbor exon 9 of PIK3CA at annealing temperature 59 °C. The exon 9 of PIK3CA gene sequences in tumor samples showed 100% similarity with the NCBI reference sequences of PIK3CA gene. The designed primer successfully amplified region for exon 9 of PIK3CA gene although no mutation found in exon 9 of PIK3CA gene in ten breast cancer patients. Keywords : Breast cancer, Mutation, PIK3CA gene, PCR, Sequencing