MANUFACTURE AND CHARACTERIZATION OF INDICATOR ELECTRODES FROM PPy + H2SO4 AND PPy + SULFONIC ACID AS A UREA SENSOR USING UREASE ENZYME IMMOBILIZATION TECHNIQUE IN PVA
| Main Authors: | Hakim S., Abdul, Rianna, Martha, Rais, Abdul |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | ind |
| Terbitan: |
Science Direct
, 2023
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| Subjects: | |
| Online Access: |
http://digilib.unimed.ac.id/49576/1/Fulltext.pdf http://digilib.unimed.ac.id/49576/2/Turnitin.pdf http://digilib.unimed.ac.id/49576/3/Koresponden%20Journal.pdf http://digilib.unimed.ac.id/49576/ https://doi.org/10.1016/j.mset.2022.11.010 |
Daftar Isi:
- esearch has been carried out on indicator electrodes (1) PVA-Enzyme/PVC-KTpClPB, sensitivity 19,069 mV/decade, detection range 1.10−5–5.10−4 M, detection limit 1.10–5 M. The width of the peak UV–vis absorbance is narrow (2) PVA-Enzyme/GA-2.9 %/PVC-KTpClPB wide UV–vis absorbance peak but the absorbance peak decreased, (3) PVA-Enzyme/GA-2.9 %/PVC-KTpClPB-o-NPOE XRD analysis amorphous spectral pattern appeared (4) PVA-Enzyme/GA-2.9 %/PPy + H2SO4/PVC-KTpClPB-o-NPOE (5) PVA-Enzyme/GA-2.9 %/PPy + Sulfonic Acid/PVC-KTpClPB-o-NPOE, amorphous spectrum pattern in (4) and (5) were greatly reduced for the enzyme variation of 0.6 g in 0.5 mL (50 % water + 50 % alcohol). GA plays a role in increasing the detection range, o-NPOE forms amorphous, enzyme variations increase the intensity of the XRD spectrum pattern. The method of developing a gradual modification of the indicator electrode membrane by cross-linking GA, o-NPOE, conductive polymer. The best results were obtained at the indicator electrode PVA-Enzyme/GA-2.9 %/PPy + Sulfonic Acid/PVC-KTpClPB-o-NPOE. Analysis of the linear curve of the sample EI5-1 with a sensitivity of 41.56 mV/decade, a detection range of 10−4–10−1 M and a detection limit of 10−4 M, R2 = 97.51 %. The best indicator electrode is EI5-1.