ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC BACTERIA ISOLATED FROM COTYLELOBIUM MELANOXYLON (HOOK.F.) PIERRE

Main Author: Idramsa,
Format: Article PeerReviewed Book
Bahasa: ind
Terbitan: JP. Research Publication , 2016
Subjects:
Online Access: http://digilib.unimed.ac.id/41211/1/PEER%20REVIEWER.pdf
http://digilib.unimed.ac.id/41211/2/TURNITIN.pdf
http://digilib.unimed.ac.id/41211/
https://ijpbs.net/abstract.php?article=NTExMw==
ctrlnum 41211
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://digilib.unimed.ac.id/41211/</relation><title>ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC BACTERIA ISOLATED FROM COTYLELOBIUM MELANOXYLON (HOOK.F.) PIERRE</title><creator>Idramsa, </creator><subject>QR Microbiology</subject><subject>QR180 Immunology</subject><subject>QR75 Bacteria</subject><description>The current study used the culturable endophytic bacterium Nocardiopsis sp. BER-17 isolated from the barks of Cotylelobium melanoxylon to synthesize a novel antimicrobial compounds. Cotylelobium melanoxylon is one of therapeutic plant species used in traditional medicine. It is containing compounds with antibacterial activity and bacterial endophytes that provide bioactive metabolites for medicinal purposes, and sources of novel compounds. An attempt has been made to employ endophytic bacterium isolate from medicinal plants in the synthesis of antibacterial compounds. The objectives of this study was to investigate the antibacterial activities produced by Nocardiopsis sp. BER-17. Dichloromethane endophytic bacterial extract and culture broth were tested for antimicrobial activity using well diffusion method against pathogenic bacteria of Escherichia coli ATCC 35218, Staphylococcus aureus ATCC 25923, Bacillus subtilis FNCC 0061 and Pseudomonas aeruginosa FNCC 0063. The antibacterial activities were assessed based by the presence of clear zones as inhibition zones. Composition of secondary metabolites in the dichloromethane bacterial extract was spectrometrically analyzed using GC-MS. The endophytic bacterial extract activity produced with clear zones against both Gram negative and Gram positive bacterial pathogens. Based on GC-MS result, dichloromethane extract endophytic contain components antibacterial activity.</description><publisher>JP. Research Publication</publisher><date>2016-04</date><type>Journal:Article</type><type>PeerReview:PeerReviewed</type><type>Book:Book</type><language>ind</language><identifier>http://digilib.unimed.ac.id/41211/1/PEER%20REVIEWER.pdf</identifier><type>Book:Book</type><language>ind</language><identifier>http://digilib.unimed.ac.id/41211/2/TURNITIN.pdf</identifier><identifier> Idramsa, (2016) ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC BACTERIA ISOLATED FROM COTYLELOBIUM MELANOXYLON (HOOK.F.) PIERRE. International Journal of Pharma and Bio Sciences, 7 (2). pp. 666-672. ISSN 0975-6299 </identifier><relation>https://ijpbs.net/abstract.php?article=NTExMw==</relation><recordID>41211</recordID></dc>
language ind
format Journal:Article
Journal
PeerReview:PeerReviewed
PeerReview
Book:Book
Book
author Idramsa,
title ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC BACTERIA ISOLATED FROM COTYLELOBIUM MELANOXYLON (HOOK.F.) PIERRE
publisher JP. Research Publication
publishDate 2016
topic QR Microbiology
QR180 Immunology
QR75 Bacteria
url http://digilib.unimed.ac.id/41211/1/PEER%20REVIEWER.pdf
http://digilib.unimed.ac.id/41211/2/TURNITIN.pdf
http://digilib.unimed.ac.id/41211/
https://ijpbs.net/abstract.php?article=NTExMw==
contents The current study used the culturable endophytic bacterium Nocardiopsis sp. BER-17 isolated from the barks of Cotylelobium melanoxylon to synthesize a novel antimicrobial compounds. Cotylelobium melanoxylon is one of therapeutic plant species used in traditional medicine. It is containing compounds with antibacterial activity and bacterial endophytes that provide bioactive metabolites for medicinal purposes, and sources of novel compounds. An attempt has been made to employ endophytic bacterium isolate from medicinal plants in the synthesis of antibacterial compounds. The objectives of this study was to investigate the antibacterial activities produced by Nocardiopsis sp. BER-17. Dichloromethane endophytic bacterial extract and culture broth were tested for antimicrobial activity using well diffusion method against pathogenic bacteria of Escherichia coli ATCC 35218, Staphylococcus aureus ATCC 25923, Bacillus subtilis FNCC 0061 and Pseudomonas aeruginosa FNCC 0063. The antibacterial activities were assessed based by the presence of clear zones as inhibition zones. Composition of secondary metabolites in the dichloromethane bacterial extract was spectrometrically analyzed using GC-MS. The endophytic bacterial extract activity produced with clear zones against both Gram negative and Gram positive bacterial pathogens. Based on GC-MS result, dichloromethane extract endophytic contain components antibacterial activity.
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