UJI AKTIVITAS ANTIBAKTERI EKSTRAK N-HEKSANA, ETIL ASETAT, DAN METANOL DAUN PERIA LAUT (COLUBRINA ASIATICA (L.) BRONGN) TERHADAP PERTUMBUHAN BAKTERI METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)
| Main Author: | AYANATUN HUSNA |
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| Format: | |
| Online Access: |
http://etd.unsyiah.ac.id//index.php?p=show_detail&id=35639 |
Daftar Isi:
- ABSTRAK Resistensi antibiotik terhadap bakteri mendorong dilakukannya berbagaipenelitian untuk mendapatkan bahan baku obat yang berasal dari alam sebagaiantibakteri. Penelitian ini menguji aktivitas antibakteri ekstrak n-heksana, etilasetat, dan metanol daun peria laut (Colubrina asiatica (L.) Brongn) terhadapbakteri Methicillin Resistants Staphylococcus aureus (MRSA). Ekstraksi daunperia laut dilakukan dengan metode maserasi bertingkat menggunakan ekstrak n-heksana, etil asetat, dan metanol. Terhadap masing-masing ekstrak dilakukan ujifitokimia dan karakterisasi ekstrak. Uji aktivitas antibakteri dilakukanmenggunakan metode Kirby-Bauer dengan variasi konsentrasi 5, 10, 15, dan 20%.Hasil uji fitokimia menunjukkan bahwa ekstrak n-heksana tidak memberikan hasilpositif terhadap golongan senyawa alkaloid, saponin, tanin, flavonoid, dansteroid/triterpenoid. Ekstrak etil asetat mengandung senyawa saponin dan steroid.Ekstrak metanol mengandung senyawa tanin, flavonoid, saponin dan steroid.Hasil karakteristik ekstrak n-heksana, etil asetat dan metanol menunjukkan kadarair sebesar 6,70; 32,11; dan 16,52%, kadar sari larut air sebesar 4,31; 2,65; dan16,52%, kadar sari larut etanol sebesar 8,28; 56; dan 54,52%, kadar abu totalsebesar 0,14; 6,37; dan 4,83%. Hasil uji aktivitas antibakteri menunjukkan bahwaekstrak n-heksana dan metanol tidak memiliki zona hambat, sedangkan ekstraketil asetat menunjukkan zona hambat terbesar pada konsentrasi 20% dengan rataratadiameterzonahambat10,49mm.Katakunci: Colubrina asiatica (L.) Brongn, Methicillin Resistants Staphylococcus aureus, dan metode Kirby-Bauer. ABSTRACT Antibiotic resistence on Bacterial encourages varicus research to find raw materialdrugs from nature as antibacterial. Antibacterial activity test of n-hexane, ethylacetate, and methanol extract of peria laut leaves was conducted againtsMetichillin Resistant Staphylococcus aureus (MRSA). Extraction wasaccomplished using solvent multistep maceration method by n-hexana, ethylacetate, and methanol. The charactetization of extract and phytochemical test wereevaluated for peria laut leaves extract. Antibacterial activity was determined byKirby-Bauer method with variation of concentration 5, 10, 15, and 20%.Phytochemical test results showed that n-hexane extract was not give positiveresult on alkaloid, saponin, tannin, flavonoid and steroid/triterpenoid. Ethylacetate extract contains saponin and steroid compound. Methanol extract containstannin, flavonoid, and steroid. Characterization extract n-hexane, ethyl acetate,and methanol extract showed that water content 6.70; 32.11; and 16.52%, watersoluble extract were 4.31; 2.65; and 54.52%, ethanol soluble extract 8.28; 56.26;and 54.53%, and total ash value 0.14; 6.37; and 4.83%. The results of antibacterialactivity test showed that n-hexane and methanol extract were not presentinhibition zone, however ethyl acetate extract showed that the largest inhibitionzone on 20% concentration with average diameter was 10,49 mm. Keywords: Colubrina asiatica (L.) Brongn, Metichillin Resistant Staphylococcus aureus, and Kirby-Bauer method.