Biji Labu Kuning (Cucurbita moschata ex Poir) sebagai Peningkat Antioksidan pada Tempe
Main Author: | Pabesak, Rivy Valen |
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Other Authors: | Dewi, Lusiawati, Lestario, Lydia Ninan |
Format: | Thesis application/pdf |
Bahasa: | ind |
Terbitan: |
Program Studi Kimia FSM-UKSW
, 2016
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Subjects: | |
Online Access: |
http://repository.uksw.edu/handle/123456789/8433 |
Daftar Isi:
- Pumpkin seeds contain phenolic useful as an antioxidant. Utilization of pumpkin seeds to increase antioxidant in tempe has not been studied in Indonesia. Therefore, this study aimed to determine the antioxidant activity, total phenolic content, water content in tempe with the addition of pumpkin seeds powder (Cucurbita moschata ex Poir), the IC50 value in pumpkin seed powder, and organoleptic testing. The percentage of pumpkin seed powder which is added to tempe are 0 ; 2,5 ; 5 ; 7,5 ; and 10%. Antioxidant activity in tempe and IC50 value in pumpkin seed powder was measured by DPPH method (2,2-diphenyl-1-pikrilhidrazil), total phenolic content measured by Folin-Ciocalteu method. The water content was measured by the oven method and organoleptic tests carried out on appearance, aroma, flavor, and texture of the 30 panelists. The results showed that the antioxidant activity and total phenolic content in tempe increased with the increasing of the pumpkin seed powder percentage that was added. Antioxidant activity and total phenolic content in tempe with different percentage of pumpkin seeds addition in a row ranged from 87,33 ± 0,93 to 91,21 ± 0,78% and 0,11 ± 0,02 to 0,14 ± 0,02 mg gallic acid / g sample. Samples for water content 0% to 10% decrease from 56,23 ± 5,51% to 51,72 ± 0,63%. IC50 values in the pumpkin seed powder obtained a value of 0,1140 g / ml. Organoleptic test showed that the most preferred tempe in terms of taste is a tempe with 10% addition of pumpkin seed powder (score of 3,43 out of 5), in terms of texture at 7,5% (score of 3,3 out of 5). In terms of appearance and aroma, the panelists gave a score that was not significantly different for all treatments.