Studi Distribusi dan Komposisi Pigmen Fotosintetik pada Tiga Species Rumput Laut Coklat yang Tumbuh di Perairan Madura
| Main Author: | Indrawati, Renny |
|---|---|
| Other Authors: | Limantara, Leenawaty, Susanto, A.B. |
| Format: | Thesis application/pdf |
| Bahasa: | eng |
| Terbitan: |
Magister Biologi Program Pascasarjana UKSW
, 2013
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| Subjects: | |
| Online Access: |
http://repository.uksw.edu/handle/123456789/1441 |
Daftar Isi:
- Lembar Pengesahan tidak disertai tanda tangan dosen pembimbing
- Brown seaweeds (Phaeophyta) are one of the abundant marine resources in Madura waters. Brown seaweeds are particularly rich in carotenoids, especially in fucoxanthin, and also in chlorophyll a. Sargassum sp. has a special morphology among Phaeophyta division due to its highly differentiated thallus form which can be divided into three main parts, namely stem-like, leaf-like and vesicle. The aim of this study was to determine the dominant pigments distribution and composition in the three main parts of Sargassum filipendula C. Agardh, Sargassum polycystum C. Agardh and other Sargassum spp. which were collected from Madura waters. The absorption spectra of acetone extract were determined using UV-1700, UV-Vis Spectrophotometer (Shimadzu, Kyoto) in order to predict qualitatively the dominant pigments of each part. The pigment extract was separated by reversed-phase high-performance liquid chromatography with Shim-pack VP-ODS C-18 column using an elution gradient of methanol, acetone and ammonium acetate solution. The dominant pigments have been detected as chlorophyll a and fucoxanthin. The results show that vesicles have the highest chlorophyll a content in all species, while the fucoxanthin content in each species has different distributions.