THE EFFECTIVENESS OF COLCHICINE AND ORYZAL IN ON POLYPLOIDY INDUCTION IN TEAK (Tectona grandis Linn. f.) IN VITRO
| Main Authors: | Fauzan, Yusuf Sigit Ahmad, Supriyanto, Supriyanto, Mulyono, Joko, Tajuddin, Teuku |
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| Format: | Article info eJournal |
| Bahasa: | eng |
| Terbitan: |
Secretariat of Agency for Standardization of Environment and Forestry Instruments
, 2024
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| Subjects: | |
| Online Access: |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/IJFR/article/view/7930 http://ejournal.forda-mof.org/ejournal-litbang/index.php/IJFR/article/view/7930/6008 |
Daftar Isi:
- The Indonesian government has launched the rehabilitation of a community plantation forest program in the entire country that would be beneficial for remedying the shortage in domestic demand for teak wood every year. This program needs to be supported by the availability of quality seed resources and quality seedlings utilizing polyploid teak (Tectona grandis). Our study aimed to examine the effectiveness of colchicine and oryzalin to modify diploid into polyploid T. grandis based on growth response, morphological, anatomical, and cytological alteration, as well as the acclimatization ability of polyploid plantlets. The materials used were aseptic lateral shoots consisting of nodal segments immersed in antimitotic agents at the concentrations of 0, 15, and 30 μM for 5 days, then cultured on regeneration medium until the 8th week and followed by acclimatization. The results showed that colchicine at a concentration of 30 μM was the most effective to induce polyploidy of plantlets in the parameter of high growth rate, length of internodes, number of leaf plantlets, leaf surface area, and significant chlorophyll index content compared to the control. Anatomical analysis of polyploidy was characterized by increasing leaf thickness, stomata size, decreased stomatal density, and increased chloroplast content in guard cells. Based on the cytological examination of polyploidy plantlets, there was an increase in the number of chromosomes in the cell nucleus. The acclimatization of polyploid plantlets successfully induced rooting and 100% survival rate of grown plantlets. Polyploid seedlings were able to grow and well adapted to the environment condition of acclimatization