PENGARUH PEMBERIAN KOMBINASI 5FULEUCOVORIN DENGAN PHALERIA MACROCARPA TERHADAP PROLIFERASI SEL DAN DIAMETER ADENOKARSINOMA KOLON TIKUS SPRAGUE DAWLEY
Daftar Isi:
- Introduction The incidence of colon cancer in the world is still high and the most cause of death of non-communicable disease category. Surgery remains the top choice therapy with other modalities such as chemotherapy, radiation and immunotherapy as P macrocarpa (Mahkota Dewa). This study seeks to prove the effects of fruit extracts P macrocarpa against colon adenocarsinoma, especially in terms of cell proliferation and changes in diameter of the tumor mass. Method Laboratory experimental research with post-test only design. This study used a female Sprague dawley white rats were divided into 4 groups : group K, P1 (chemotherapy group), P2 (P macrocarpa group), and P3 (combination group). Colon tumors induced by 1,2-DMH subcutaneously.Chemotherapy given 5FUleucovorin as much as 6 cycles according Rosswell Park Regiment. P macrocarpa given at a dose of 0,495 mg / day (0.99 mL / day) orally. Cell proliferation was assessed by IHC Ki-67 staining, while the diameter of the tumor mass was measured using a caliper tumor. Result Mean the cell proliferation and diameter of tumor masses on K, P1, P2, P3 group was 37.150 ± 8.878, 28.567 ± 12.531, 35.533 ± 8.982, 22.567 ± 3.445 and 1.403 ± 0.265, 1.135 ± 0.154, 1.339 ± 0.111, 1.074 ± 0.164 respectively. Statistical analysis showed a significant difference in cell proliferation between Kvs P3 (p=0.011), P2 vs P3 (p=0.022) and significant difference in diameter of the tumor mass between K vs P1 (p=0.020), K vs P3 (p=0.005), P2 vs P3 (0.021). Correlation analysis of cell proliferation with a diameter of the tumor mass was not significant (p=0.405). Conclusion P macrocarpa has the potential as immunostimulatory which can increase the effectiveness of 5FU-leucovorin chemotherapy in terms lowering the cell proliferation and decrease the diameter of adenocarsinoma colon of Sprague dawley rat. Keywords : P macrocarpa, colon adenocarsinoma, cell proliferation, tumor mass diameter