PENGARUH PEMBERIAN EKSTRAK PHALERIA MACROCARPA TERHADAP EKSPRESI IFN- ɣ DAN SEBUKAN SEL MONONUKLEAR PADA MENCIT SWISS DENGAN KARSINOMA EPIDERMOID KULIT
Daftar Isi:
- Backgroud. Incidence of Epidermoid skin cancer is high. Phaleria macrocarpa in previous research can stimulate immunity reponse. Objective. To know the response of supplementation of Phaleria Macrocarpa (PM) to Paclitaxel–cysplatin (Pacli-Cys) in the treatment of Swiss mice with epidermoid skin cancer. Methods. Post test only control group design in 12 Swiss mice, who were successfully initiated with 7,12-dimethylbenzanthracene (DMBA) and promoted with 12-O-tetradecanoyl phorbol-13-acetate (TPA) were randomly allocated into 4 groups: without treatment, treated with PM, treated with Pacli-Cys ,and treated with Pacli-Cys + PM. Tumor removed and prepared for pathologic examination using Mouse Monoclonal Ig G IFN-γ for imunohistochemistry staining, and hematoxylin eosin staining. Result. Supplementation of PM and or Pacli-cys didn’t increase expression of IFN-γ compared with without treatment group . Supplementation of PM increase expression tumor-infiltrating mononuclear cell compared with without treatment group (p=0,05). Suplementation Pacli-Cys significantly increase expression of tumor- infiltrating mononuclear cell compared with without treatment(p=0,05). Supplementation Pacli-Cys + PM significantly increase tumor-infiltrating mononuclear cell compared with without treatment group (p=0,05). The correlation between the expression of IFN-γ and tumor-infiltrating mononuclear cell was significantly positive (p = 0,033 dan r = 0,616). Conclusion. PM and or Pacli-Cys supplementation has no effect on stimulating Interferon-γ production. PM and or Pacli-Cys supplementation has synergic effect on stimulating tumor-infiltrating mononuclear cell. PM supplementation increasing tumor-infiltrating mononuclear cells higher than Pacli-cys. PM+Pacli-Cys did not increase tumor-infiltrating mononuclear cell compare with PM alone or Pacli-Cys alone. Key words: Epidermoid Skin Cancer- Phaleria Macrocarpa-Paclitaxel-Cysplatin- Interferon γ-Tumor Infiltrating Mononuclear Cells