Effect of Phenylacetic Acid Addition on Productivity of Penicillium chrysogenum in Penicillin G Production Using Pilot Scale Reactor
| Main Authors: | Amila , Pramisandi, , Rofiq , Sunaryanto,, S, Suyanto, , Erwahyuni, Endang Prabandari |
|---|---|
| Format: | Proceeding PeerReviewed application/pdf |
| Terbitan: |
, 2012
|
| Subjects: | |
| Online Access: |
http://eprints.undip.ac.id/36934/1/BRE-15_Amila.pdf http://eprints.undip.ac.id/36934/ |
Daftar Isi:
- Effect of penicillin’s precursor addition, phenylacetic acid (PAA), was done by addition of PAA in several concentrations in a pilot scale reactor batch culture to produce penicillin G with fungi Penicillium chrysogenum. Culture was analyzed by morphology observation and PAA was analyzed by HPLC. High concentration of PAA showed decreasing of biomassa and production of penicillin G that had contribution in increasing of cellular autolysis. However, Penicillium chrysogenum’s morphology analysis did not show significant effect in autolysis and decreasing of biomassa. Low concentration of PAA showed low production of penicillin G and low effect in biomassa or autolysis. Those effects of PAA addition need an exploitation to induce the phenomenon in Penicillium chrysogenum’s culture.