The Viability and Membrane Integrity of Spotted Buffalo Epididymal Sperm in Addition of Maltose into Andromedï¢Extender
| Main Authors: | Herdis, Herdis, Surachman, M, Yulnawati, Yulnawati, Rizal, M, Maheshwari, H |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
Faculty of Animal Agriculture, Diponegoro University
, 2008
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| Subjects: | |
| Online Access: |
http://eprints.undip.ac.id/18998/1/33(2)2008p101-106.pdf http://www.fp.undip.ac.id http://eprints.undip.ac.id/18998/ |
Daftar Isi:
- The aim of this study was to investigate the viability and membrane integrity of spotted buffalo epididiymal sperm. Andromedï was used as control extender and the combination of Andromedï with 0.2% (P1) and 0.4 % (P2) maltose were used for the treatments. The results showed that the percentage of motility after thawing in P1 media (46.00 ï± 2.00 %) was not significantly different (P>0.05) from P2 (44.00 ï± 2.00 %), but there was significantly different (P<0.05) from K (41.00 ï± 2.00 %). The percentage of live sperm after thawing in P1 (59.20 ï± 1.17 %) was not significantly different (P>0.05) from P2 (60.60 ï± 1.62 %), but significantly different (P<0.05) from K (52.20 ï± 2.48 %). There was no significantly different (P>0.05) in the percentage of membrane integrity of spotted buffalo epididymal sperm at the three stages of cryopreservation process in the three different extenders. In conclusion, the addition of maltose into Andromedï could maintain the viability and membrane integrity of spotted buffalo epididymal sperm after thawing. Keywords: Viability, Plasma membrane, Epididymal sperm, Maltose, Spotted buffalo