ANTIBACTERIAL ACTIVITY OF A SECONDARY METABOLITE-PRODUCING CORAL BACTERIUM Pseudoalteromonas SPECIES
| Main Authors: | Karna Radjasa, Okky, Marten, Torben, Brinkoff, Thorsten, Grossart, Hans-Peter , Sabdono, Agus, Simon, Meinhard |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
Research Institute Diponegoro University
, 2004
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| Subjects: | |
| Online Access: |
http://eprints.undip.ac.id/146/1/Antibactery_activity_of_a_secondary_metabolite.pdf http://www.coastdev.undip.ac.id http://eprints.undip.ac.id/146/ |
Daftar Isi:
- A bacterium, collected at the surface of coral Acropora sp., TAB4.2 was successfully screened for secondary metabolites production based on PCR amplification of the non-ribosomal peptide synthetase gene. It was identified as closely related to Pseudoalteromonas luteoviolacea based on its 16S rDNA. TAB4.2 was found to inhibit the growth of all 5 coral-associated and all 5 pathogenic bacteria tested. To characterize the inhibiting metabolite, a 279 bp long DNA fragment was obtained and the deduced amino acid sequence showed conserved signature regions for peptide synthetases and revealed a high similarity to NosD (40 % identity), a multifunctional peptide synthetase from Nostoc sp. GSV224, and NdaB (44 % identity), a peptide synthetase module of Nodularia spumigena.