Peran Limfosit T Helper-1 (TH1) dan T Helper-2 (TH2) pada Patogenesis Artritis Lepra
| Main Authors: | Hadi, Suyanto , Suyanto , Suyanto , S, Hardyanto, Triyuliati, Triyuliati, J, Susanto , Hartono,, F.X. |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
Fakultas Kedokteran Universitas Diponegoro
, 2009
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| Subjects: | |
| Online Access: |
http://eprints.undip.ac.id/14107/1/vol_43_no_1_2008_hal_44_53.pdf http://www.mediamedika.net http://eprints.undip.ac.id/14107/ |
Daftar Isi:
- The role of lymphocyte T Helper-1 (TH1) and T Helper-2 (TH2) in the pathogenesis of leprosy arthritis Background: The autoreactive of TH CD4+ cells is the thought to play an important role in arthritis leprosy pathogenesis. However, wheter of TH1 or TH2 predominant has never been studied. Methods: Various Ag M. leprae (Ag 35 kDa, 10 kDa, 45 kDa, 85 kDa, and MLSA 2 ug/ml) were stimulated to the peripheral blood (10cc) lymphocyte culture (PBMC) using 96 weels microplate and RPMI 1640 media of 22 leprosy arthritis cases, control-1 (n=12) (leprosy without arthritis) and control 2 (n=12) (healthy contact). The activity difference between TH1 and TH2 CD4+ lymphocyte was analysed using the difference delta levels of IFN- and IL-4 (ELISA) of the three group studies. Statistical analysis used were ANOVA, Kruskal Wallis or Mann Whitney, and Chi-square. Results: IFN- delta levels was significantly higher in the lymphocytes cultures in LA group (the median 132.234 pg/ml, 60.347 pg/ml, 14.093 pg/ml, 16.619 pg/ml and 138.394 pg/ml) compared with IL-4 value level (median 0.317 pg/ml, 0.017 pg/ml, -0.206 pg/ml, -0.200 pg/ml and 0.492 pg/ml) after being stimulated with 35 kDa, 10 kDa MMP-1, 45 kDa LAM, 85 kDa and MLSA of 2 ug/ml dose consecutively (all p<0.001). The IFN- delta value in LA group also showed the significantly higher level in response to all M. leprae Ag compared to all control-groups, with all p value < 0.05. Conclusion: TH1 CD4+ lymphocyte activity is more dominant compared than TH2 CD4+ lymphocyte activity in leprosy arthritis group patients