Isolation and Analysis of Sucrose Synthase (SUS) Gene Fragment Originated from “Kopyor” Coconut Mutant
| Main Authors: | Sukendah, Sukendah, Hugo, Volkaerd, Sudarsono, Sudarsono |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
American Scientific Publishers
, 2017
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| Subjects: | |
| Online Access: |
http://eprints.upnjatim.ac.id/7553/1/s125%2Dsukendah%2D1.pdf http://eprints.upnjatim.ac.id/7553/ |
Daftar Isi:
- Kopyor” coconut is a naturally occurring coconut mutant having abnormal endosperm. The content of sucrose in the “Kopyor” coconut endosperm is twice as much as normal one. Fragment of sucrose synthase (SUS) gene, one coding for sucrose synthase enzyme involved in the sucrose biosynthesis, was isolated from “Kopyor” coconut and its sequence were analyzed. The size of DNA fragments of SUS gene originated from “Kopyor” coconut (CnSus1A, CnSus1B, CnSus2A, and CnSus2B) were 746, 738, 780, and 687 bp, respectively. Analysis of DNA sequences of CnSus1A, CnSus1B, CnSus2A, and CnSus2B showed features of non-grass monocotyl of SUS gene. Gene specific primer pairs (CnSUS1A, CnSUS1B, CnSUS2A, and CnSUS2B) were designed based on sequences of SUS gene fragments and they could be used for analyzing variability of SUS gene among normal and mutant coconut. Keywords: Sucrose Biosynthesis Gene, Gene Specific Primers, Coconut Mutant