Kopling Oksidatif Eugenol Menggunakan Ekstrak Enzim Peroksidase dari Akar Tanaman Sawi Hijau serta Uji Bioaktivitasnya
| Main Authors: | Ardiansah, Bayu, Cahyana, Antonius Herry, Suwarso, Wahyudi Priyono, Maryana, Rianti, Merly, Siti |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan
, 2018
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| Subjects: | |
| Online Access: |
http://ejournal.uin-malang.ac.id/index.php/Kimia/article/view/3776 http://ejournal.uin-malang.ac.id/index.php/Kimia/article/view/3776/5603 |
Daftar Isi:
- Peroxidase has been successfully extracted and partially purified from Brassica juncea L. Czern root with protein content and specific activity of 0.747 mg/mL and 0.244 U/mg, respectively. Then, it was applied as biocatalyst for green oxidative coupling reaction of eugenol to produce an orange crystal, dimeric form of eugenol (84% yield). Structure elucidation was done by using FTIR, UV-Vis and GC-MS instruments. The optical activity of dimeric eugenol was found to have Ra-configuration. Furthermore, biological activity of the product as antioxidant has been evaluated resulting in IC50 values of 32.14 and 42.43 ppm for dimeric and monomeric eugenol, respectively.