Extraction, Toxicity Assay and Identification of Active Compounds of Red Algae (Eucheuma cottonii) from Sumenep Madura
| Main Authors: | Afif, Sholeh, Fasya, Ahmad Ghanaim, Ningsih, Rachmawati |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan
, 2016
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| Online Access: |
http://ejournal.uin-malang.ac.id/index.php/Kimia/article/view/3199 http://ejournal.uin-malang.ac.id/index.php/Kimia/article/view/3199/5047 |
Daftar Isi:
- Extraction, Toxicity Assay and Identification ofActive Compounds of Red Algae Extract (Eucheuma cottonii) from Tanjung Sumenep Madura was analysed. Extraction of active compound was conducted by maceration method using methanol as a solvent. The methanol extract was hydrolyzed with HCl 2 N. The result was partitioned using five solvents (1-butanol, ethyl acetate, chloroform, petroleum ether and n-hexane). Toxicity level was determined by Brine Shrimp Lethality Test (BSLT). The identification of the active compound was analyzed by phytochemical test, qualitatively. The result showed that the 1-butanol is the best solvent to extract active compounds of red algae E. Cottonii in partition process. 1-Butanol fraction gives the highest activity of toxicity with LC5070,32 ppm. LC50 value for ethyl acetate, chloroform, petroleum ether and n-hexane was 143,43; 194,40; 195,28; 303,66; and 634,97 ppm, respectively. Identification of active compounds indicated that 1-butanol fraction contains steroids and alkaloids. Keywords: Eucheuma cottonii, extraction, Brine Shrimp Lethality Test, phythochemical