SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR)
| Main Authors: | , Agus Riyanto, , Dr. Edi Suharyadi |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/99817/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56173 |
Daftar Isi:
- Fe3O4 nanoparticles have been successfully fabricated by co-precipitation method. To study the potency of Fe3O4 as active material on surface plasmon resonance based biosensor application, the magnetic properties, modifying the surface of Fe3O4 nanoparticles by making a single layer system using polyethylene glycol 4000 (PEG-4000), and binding of the modified nanoparticles to biomolecules (α-amylase) have been also investigated. Fe3O4 nanoparticles was synthesized from 1 mol FeSO4.7H2O and 2 mol FeCl3.6H2O by co-precipitation method by controlling the synthesis temperature, concentration of co-precipitan (NH4OH), and duration of centrifugation. The results of x-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis showed that Fe3O4 nanoparticles with the smallest grain size and low levels of impurities can be obtained by increasing the concentration of co-precipitan, and holding of temperature synthesis at room temperature (about 30 o C), and to extend the duration of centrifugation. The result of measurement using vibrating sample magnetometer (VSM) showed that the smaller the grain size (diameter) Fe3O4 nanoparticles, the higher the magnetic response in the nanoparticles. In addition, the smaller the grain diameter of nanoparticles Fe3O4 nanoparticles coercivity also decreases. Analysis of infra-red spectroscopy (IR spectroscopy) showed that the surface of Fe3O4 nanoparticles can be modified with PEG-4000 through a covalent bond Fe-O. Modification of Fe3O4 nanoparticles was proven to be increase dispersibility nanoparticles in a liquid. In addition, the results of IR spectroscopy analysis showed that PEG-4000 layer can bind to the target biomolecules (α- amylase). Thus, in particular Fe3O4 nanoparticles with very small grain size has a great potency can be harnessed to improve the immobilization of biomolecules on the sensing surface of SPR based biosensor.