Induksi Ketahanan Anggrek Phalaenopsis Terhadap Penyakit Busuk Lunak dengan Introduksi Bakteri Endosimbion
| Main Authors: | , DEWI NUR ANGGRAENI, , Dr. Budi Setiadi Daryono, M.Agr.Sc. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/99104/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=55464 |
Daftar Isi:
- Orchid is ornament plant with beautiful flower and become a potential income source of the country. Soft rot disease is one of problem in orchid cultivation. This disease is caused by bacteria, e.g., Pseudomonas vinidiflava, P. carotovorum, and Dickeya dadantii. Orchid infected by soft rot shows brown spots on leaves, rotting tissue, and unpleasant odor. Biological disease control on orchid was rarely done. Endosymbiotic bacteria was suspected to induce Phalaenopsis resistance to soft rot disease. This research was aimed to identify endosymbiont bacteria which is able to inhibit the growth of soft rot bacteria and its relationship with other bacteria, and analyze the resistance of endosymbiotic bacteria introduced Phalaenopsis to soft rot disease. Survey and sample collection were done at Wonosadi Forest and nurseries in Yogyakarta and West Java. One hundred and six isolates of endosymbiotic bacteria were obtained. From antagonistic test, one isolate (TbPh7) which had highest inhibition effects and potential in soft rot disease inhibition was chosen. Deoxyribonucleic acid (DNA) extraction of TbPh7 isolate was done by cetyl trimethyl ammonium bromide (CTAB) method and amplified by polymerase chain reaction (PCR) method. Amplified DNA fragment was located around 900 bp. Phylogenetic analysis showed that TbPh7 isolate was belong to Bacillus sp. group. Resistance test showed increase of Phalaenopsis resistance to soft rot disease after endosymbiotic bacteria introduction. Treatment III (soaking+spraying) had the smallest disease intensity value.