ANALISIS DINAMIKA GENETIK GEN NS1 VIRUS AVIAN INFLUENZA H5N1 ISOLAT BALI
| Main Authors: | , ARIEF MULYONO, , Prof. drh. Widya Asmara, SU, Ph.D. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/98673/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=55567 |
Daftar Isi:
- Background. H5N1 virus is estimated to continue to mutate potentially increasing the capacity for passing through the barrier, and spreading out easily between human. Viral transmission between human is the beginning of a global pandemic H5N1 virus. Combination of among gene fragments (genetic makeup) H5N1 virus appears to jointly determining the virulence level. Java is epicenter H5N1 viruses in Indonesia. H5N1 viruses are being introduced from Java into surrounding islands include Bali island through the poultry trade. The aim of this study were to analyze the genetic dynamics of the NS1 gene and find out the potential virulence increase of H5N1 avian influenza origin from Klungkung Bali chicken. Methods. The method used in this study were isolation of RNA, NS1 gene amplification by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), electrophoresis and sequencing. Sequence data of avians influenza H5N1 virus isolat from Klungkung Bali was then compared with a multiple aligment with other Indonesian isolat from different hospes accessed through GenBank 2005-2007. The next step after comparing the sequence was phylogenetic analysis and prediction of three dimensional structure of the NS1 protein. Results. Four isolates underwent P42S substitution and 5 amino acid deletion at the position 80 � 84 resulting in increased virulence of the virus, but there were not found the D92E, F103L and M106I substitutions. Phylogenetics analysis revealed that four test isolates had a closer genetic relationship with the virus isolates from cat and human. The isolates test had increased of nucleotide and amino acid substitusion than isolates from Bali in 2005. Conclutions. Avian influenza virus H5N1 isolates from Bali is being dynamic genetic. Virulence marker had found on NS1 gene sequence of isolates test from Bali.