BIODIVERSITAS BAKTERI TANAH PEMARASIT NEMATODA SISTA KUNING (GLOBODERA ROSTOCHIENSIS)
| Main Authors: | , Irianti Kurniasari, , Dr. Ir. Jaka Widada, M.P |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/98066/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=54286 |
Daftar Isi:
- Golden potato cyst nematode, Globodera rostochiensis, was firstly reported to be detected in Indonesia for the first time in 2003 and currently is being an important potato pest. The parasitic soil bacteria found only parasitic on cyst nematode of the genera Globodera, but not specifically on the species G. rostochiensis. This research is conducted to find out the existence and diversity of parasitic soil bacteria against G. rostochiensis. Soils taken from rhizospheric healthy and infected potato plant were used for bacterial isolation. Nutrient and soil extracts agar media used to grow the bacterial isolates. The parasitic ability of bacterial isolates were tested against cysts and eggs ofG. rostochiensis using bioassay method. The genetic diversity of the isolates was determined using PCR TP-RAPD (Polymerase Chain Reactions Two Primer Random Amplified Polymorphic DNA) technique and UPGMA (Unweighted Pair Group Method Arithmetic Average). Fourty five isolates were observed to be parasitic against cysts and eggs of G. rostochiensis. Among them, 24 and 21 isolates were from healthy and infected potato plant rhizosphere soils, respectively. Eighty and ninety eight point seven percent of total cysts and eggs, respectively, could be parasitized by the best isolate. The genetic diversity analysis showed that 33 isolates have similarity index value less than 97% and 12 isolates have similarity index value more than 97%, indicated that 73,3% of the isolates are different. It can be concluded, that the soil bacteria which are parasitic to G. rostochiensis has been evolved, as well as their diversity.