PENGEMBANGAN MURAMIDASE RELEASED PROTEIN SEBAGAI PERANGKAT DIAGNOSTIK SEROLOGIS INFEKSI Streptococcus suis serotipe 2
| Main Authors: | , CLARA AJENG ARTDITA, , Prof. Dr. drh. Siti Isrina Oktavia Salasia |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/97912/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=54396 |
Daftar Isi:
- Streptococcus suis is a bacterial pathogen that cause various disease of pigs, indicating meningitis, bronchopneumonia, arthritis, pericarditis, polyserositis and septicaemia. Streptococcus suis especially serotype 2 can infect human (zoonotic) with special symptom of meningitis. In 2008, S. suis has been isolated from synovial liquid of pig in Timika, Papua. The aim of this research was to developing muramidase released protein (MRP), an important virulence marker of S. suis, as a diagnostic tool of S. suis infection. Streptococcus suis strain P171 was used in this research, identified biochemically and genotypically by using polymerase chain reaction (PCR) to amplification the gene encoding capsular serotype 2 (cps2J gene) and the gen encoding MRP (mrp gene). The MRP antigen was extracted using lysozyme and separated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Balb/c mice were imunized with 136 kDa MRP to produce antibody against MRP and the antibody were evaluated by using enzyme linkage immunosorbent assay (ELISA). The results of the research showed that the antibody against MRP with molecular weight of 136 kDa could be produced on Balb/c mice with the absorbance value of 3,889 (at 50x dilution), then could be used to detect sera of field infected pigs in Papua 200x dilution. Antibody against MRP could detect serologically of S. suis serotype 2 of pigs in Papua with seropositive results of 40% (by using dot blot) and 50% (by using ELISA).