VARIASI GENETIK SERANGGA PENGHASIL SUTERA EMAS Cricula trifenestrata Helf. (Lepidoptera: Saturniidae) DENGAN PENANDA MOLEKULAR ISSR (Inter Simple Sequence Repeats)
| Main Authors: | , Agnes Herlina Dwi Hadiyanti, , Dr. Niken Satuti N.H., M.Sc. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/97316/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=54415 |
Daftar Isi:
- Cricula trifenestrata is a superior species of silk-producing in Java island, in addition to other species such as Attacus atlas and Antheraea. This species has a particular characteristic which produces golden silk cocoons that can be harnessed into a high-quality textile material. Differences in environmental conditions and feed plants will affect the adaptation response of each species that cause the variation or polymorphism occurring both in populations and individuals within the population. Research on the morphological characterization of wild silkworm, feed plants, habitats, and the resulting silk fibers have been carried out in Indonesia, but so far, there has been no research to uncover the genetic variation of wild silkworm especially C. trifenestrata. With genetic studies, information about the diversity among individuals within and between populations, can be known, so it can be used as a basis to support the identification and characterization of C. trifenestrata. The aims of this research was to conduct a study on genetic variation C. trifenestrata collected from several sampling locations using ISSR (Inter-Simple Sequence Repeat) molecular markers. The applied methods of the research was to collect samples (C. trifenestrata), isolation DNA genome, and analysis of genetic variation using ISSR-PCR method. Based on genetic variation analysis using 4 primers (ISSR 1, ISSR 2, ISSR 6, dan ISSR 7), it is known that the level of genetic variation and polymorphism of C. trifenestrata is high with a percentage of 98.9%. The formed dendogram showed that the 29 studied samples of C. trifenestrata separates into two major groups at the similarity level of 60%.