DETEKSI DAN KARAKTERISASI MOLEKULAR CYMBIDIUM MOSAIC VIRUS PADA ANGGREK DI INDONESIA
| Main Authors: | , Suprapty Abdullah, , Dr. Budi Setiadi Daryono, M. Agr. Sc. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/97307/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=54416 |
Daftar Isi:
- Orchid is one of most cultivated flora in Indonesia and has high species diversity. Invention of new varieties by plant breeding and orchid usage for aesthetics point increase orchid commercialization. Unfortunately it is not always be followed by prevention of disease transmission. There were reports of virus infected individual among orchid in Java. Cymbidium mosaic virus (CymMV) was one of the most frequently reported orchid infecting virus. Early symptom of infected plant are chlorotic spot or streak followed by necrosis. Virus detection based on symptom might be done but molecular detection is needed due to symptom differences on every species. The purposes of this research were to detect virus infection in orchid which live in nursery, botanical garden, national park, and natural habitat in Indonesia, to identify virus transmission in orchid, and to find out the relationship between CymMV isolates based on coat protein (CP) gene. Total sample was 66 and collected from Sumatra, Java, Bali, Borneo, and Celebes islands. Virus detection was done by serological analysis using ImmunoStrip and molecular using reverse transcription-polymerase chain reaction (RT-PCR) method. Five samples were revealed to be infected by ImmunoStrip and nine samples by RT-PCR method. Infected samples belonged to genus Phalaenopsis, Calanthe, Phaius, Flickingeria, Arachnis, Dendrobium, and Cattleya. Sequencing was done on two positive samples from nursery and nature orchid, CP-1 and CP-2 respectively. Among CymMV isolates, similarity index of CP gene sequence was 0.959-0.978 for CP-1 and 0.843-0.868 for CP-2. Phylogenetic analysis indicated a close relationship between CP-1 isolate with Korean and Indian isolate, while CP-2 isolate shared a distant relationship with other isolates and was located on new branch outside the CymMV subgroup. Host range test showed that CymMV infected chili pepper, melon, Dendrobium sp., Phalaenopsis sp., and Vanda sp., while tobacco and tomato plant were not infected by CymMV.