GENETIC DIVERSITY ANALYSIS OF STREPTOMYCETES ISOLATED FROM THE RHIZOSPHERE OF Paraserianthes falcataria (L) Nielsen BY USING DNA FINGERPRINTING (RIBOTYPING) METHOD
| Main Author: | , Langkah Sembiring |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : UIN Maulana Malik Ibrahim
, 2009
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| Online Access: |
https://repository.ugm.ac.id/95481/ http://repository.ugm.ac.id/digitasi/index.php?module=cari_hasil_full&idbuku=3299 |
Daftar Isi:
- <p>Genetic diversity of 37 representative strains of multimembered colour group of streptomycetes was analysed by using ribotyping method. Purified chromosomal DNA of test strains were digested with Bam HI restriction endonuclease. DNA fragments were separated with agarose gel electrophoresis and transferred to positively charged nylon membrane by Southern Blotting technique. The DNA fragments were hybridized with digoxigenin labelled probe (random primer labelled fragments of 5S, 16S, and 23S rRNA gene from Streptomyces violaceus TK210). The ribotype pattern of the test strains were analysed numerically to determine the similarities between the test strains using the SSM coeficient and the UPGMA algorithm. The ribotype patterns were analysed and presented as dendrograms. The 37 isolates showed a variety of pattern and that none of the bands were common to all of the strains, except between B2P1 (group 8) and A2P1 (group 9). It was also evident from the ribotype pattern that a number of organisms were particularly closely related, namely isolates C1P2 (group 1.2) and B10P3 (group 1.3), A4R2 (group 3.1) and A33R1 (group 3.2), B2P1 (group 8), A2P1 (group 9) and A46R1 (group 10). Accordingly, strains B12P1 (group 12), D19P2 (group 13) and A6P3 (group 29) were also closely related. Finally, another closely related strains were A36P1 (group 19), A5P1 (group 25), and C5P3 (group 37) as well as between strains A6P3 (group 29) and A22P2 (group 33). Therefore, it could be demonstrated that the ribotyping method could be used to differentiate as well as to analyse genetic diversity of bacterial strains.</p>