BIOACTIVITY SCREENING OF SPONGES COLLECTED FROM BUNAKEN, MENADO BY BRINE SHRIMP LETHALITY TEST AGAINST ARTEMIA SAUNA LEACH
| Main Author: | , Astuti, Puji dan Wahyuono, Subagus |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Fakultas Farmasi UGM
, 2003
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| Online Access: |
https://repository.ugm.ac.id/92865/ http://repository.ugm.ac.id/digitasi/index.php?module=cari_hasil_full&idbuku=621 |
Daftar Isi:
- <p>ABSTRACT<br /> Indonesia is one of the countries rich of natural resources. Sponges are marine. invertebrates widely<br /> found in Indonesia and known to have various bioactive compounds with unique chemical structures.<br /> This study was aimed to screen potentially bioactive extracts of sponges collected from Bunaken and identify on their toxicity level against larva Artemia salina Leach.<br /> Samples were macerated using acetone followed by partition using chioroforna and methanol. The extracts were prepared at the concentration of 100, 250, 500 and 1000 ug/ml and their toxicity was tested using Brine shrimp Lethality Test. LC,50 (ug/ml) of the extracts was calculated using probit analysis.<br /> From the 4 extracts tested, all of them showed toxicity to larva Artemia. Chloroform extract of MD-02 was found to be the most toxic with the LC,50 or 48.15 ug/ml and considered as a potential candidate for new drug research. Further characterization is still needed for the development in the future. This active sponge was then identified as Petrosfa sp.<br /> Key words: sponges, Bunaken, bioactivity, Artemia salina Leach</p>