Analisis Dinamika Genetik Receptor Binding Site Hemaglutinin Virus Avian Influenza H5N1 Isolat Bali
| Main Authors: | , R.Aj. SRI WULANDARI,dr, , Prof. drh. Widya Asmara, SU, PhD |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2011
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/91147/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=53151 |
Daftar Isi:
- Influenza virus host range restriction is partly determined by the receptorbinding specificity of the HA protein. Hemagglutinin of the avian influenza virus H5N1 evolves rapidly through antigenic shift and antigenic drift. Strains with mutated hemagglutinin may emerge, potentially changing the receptor binding preference from avian to human-type and causing pandemic. Avian influenza virus H5N1 was widely spread in Bali. Virus transmissions via poultry movement from Java Island were considered to play role. Close interaction between human, chickens, ducks and pigs in Bali could select HPAI H5N1 viruses that well adapted to multiple host and reduced interspecies transmission barrier of those viruses. The aim of this study is to analyze hemagglutinin receptor binding site dynamic evolution of two avian influenza viruses H5N1 from Klungkung, Bali isolated in 2009 and predict their potential risks to human. Chronological steps of this study consist of RNA isolation, amplification of receptor binding site sequence by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), electrophoresis, and sequencing. Receptor binding site sequence of avian influenza virus H5N1 isolates from Klungkung, Bali were analyzed using multiple alignment comparison with another Indonesian isolates from various hosts and phylogenetic tree construction. Two avian influenza virus H5N1 isolates from Klungkung, Bali showed recognition to avian receptor. However, two substitution sites, S137A and K193R were predicted to enhance α-2,6-linked receptor recognition. Finally, we conclude this finding could represent adaptation step from avian viruses to human.