Optimasi Produksi Lipase dari Aspergillus niger 65I6 pada Medium Fermentasi Bungkil Biji Jarak Menggunakan Metode Solid State Fermentation (SSF)
| Main Authors: | , WHENI HASLINAWATI, , Dr. Ir. Chusnul Hidayat |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2011
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/90979/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52789 |
Daftar Isi:
- Lipases (EC 3.1.1.3 triasilgliserol hydrolase) are lipolytic enzyme that catalyze hydrolysis ester bonds of triglycerides into glycerols and fatty acids. Lipases are industrial enzyme that have many application in food industry, cosmetics, detergents, pharmaceuticals and biodiesel. One of the limitations on use of lipases in the industry is the high cost needed to increase lipases production. Therefore, increasing the productivity of the fermentation process needs to be done by optimizing the condition of medium and fermentation conditions. Solid State Fermentation (SSF) is a fermentation method used to increase the production of lipases because it has a higher productivity when compared to the Submerged Fermentation (SMF). In addition, the use of agro-industry residues such as Jatropha curcas seed meal fermentation medium can reduce enzyme production costs and add value to a lower-priced material. Fungi are recognized as the best lipase producers and are currently the preferred option, especially Aspergillus niger, which is superior to several other microorganisms and produces lipases suitable for many applications. Response surface methodology (RSM) was applied to optimize lipase production. Experimental design consisted of four variables: Time course of enzyme production (x1), moisture content (x2) inducer (x3), inokulum (x4) and each independent variable was tested at three levels symbolized -1, 0, +1. A total of 27 runs were performed to study the selected variables. The parameters used in the RSM design were then further investigated to define the optimal process conditions. Time course of enzyme production and moisture content had significant effect on lipase production than inducer and inokulum. The interaction between two parameter gave negative significant. The best lipase actifity was attained using following condition: time cource of fermentation (seven days), moisture content (50%), inducer (2%), inokulum (2,3 x 107 spore/mL) and the lipase activity was 836,85 U/gds.