EVALUASI CEMARAN AFLATOKSIN B1 PADA PAKAN AYAM PEDAGING KOMERSIAL DI KOTA KUPANG, PROVINSI NTT DAN EFEKNYA TERHADAP HEPAR DAN ORGAN LIMFOID
| Main Authors: | , DRH DEVI YASINTHA JOICE APRIANTI MOENEK, , Prof. drh. Charles Rangga Tabbu, M.Sc., PhD, |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2011
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/90736/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52925 |
Daftar Isi:
- Aflatoxin B1 is a secondary metabolite of fungi of Aspergillus flavus, Aspergillus parasiticus, and Penicillium puberulum, which is frequently found as contaminants of feed/raw materials of poultry feed. Such compound has the toxic and carcinogenic effects that can cause damage to various tissues/organs, which can further decrease the performance of broiler, and cause various degrees of immunosuppressive effects. This study was designed to evaluate the aflatoxin B1 contamination on commercial broiler feed in Kupang City of East Nusa Tenggara Province and its effects on the liver and lymphoid organs (bursa Fabricius, thymus, and lien). The research was conducted on commercial broiler feed that is given to all broiler farms (10 farms) in Kupang City. Physical examinations included texture, color, and odor, followed by a qualitative examination using ultraviolet (UV) with White/2UV transiluminator. Analysis of aflatoxin B1 contamination in feed samples from the storage ("warehouse") of farm and left over feed on feeders was performed with the method of high performance liquid chromatography (HPLC) (Shimadzu type 6.1). Serological testing to determine antibody titers against Newcastle disease virus (NDV) was conducted on 20 samples of chickens from each farm (total 10 farms). After blood sampling, the same chicken (20 chickens from each farm) were killed by air embolism into the heart, then necropsy were perfomed for pathological examination. Sample of liver, bursa Fabricius, thymus, and lien were taken for further processing according to the staining method of haematoxylin and eosin (H & E). The results of physical examination of feed and pathological examination of tissues will be analyzed descriptively, whereas the contamination levels of aflatoxin B1 in feed and the antibody titer against NDV will be analyzed statistically using t-test. Based on the results of this experiment, it can be concluded that the texture of feed in the storage (�warehouse�) of farms was not changed (crumble form), whereas the left over feed indicated an irregular texture, which was crushed, moist, lumpy, sour-smelling, and glowing on irradiation with UV light. Statistical analysis using t-test showed no significant difference (P>0.05) in the level of aflatoxin B1 among of feed samples from the storage and left over feed. The results of statistically analyzes using t-test showed that antibody titres against NDV in group of chickens from farms with Aflatoxin B1 contaminated feed is not associated with the level of aflatoxin B1 in feed samples. Pathological examination on liver showed an early-stage of liver cirrhosis, atrophy of bursa Fabricius, thymus, and lien due to the necrosis and depletion of lymphocytes.