PENGARUH KOMBINASI PUPUK ANORGANIK DAN CASEIN HYDROLISATE PADA PERTUMBUHAN IN VITRO EMBRIO ANGGREK Spathoglottis plicata Blume
| Main Authors: | , TRI SARTIKONINGSIH,S.P., , Dr. Endang Semiarti, M.S. M.Sc. |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2011
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/90695/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=53020 |
| ctrlnum |
90695 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/90695/</relation><title>PENGARUH KOMBINASI PUPUK ANORGANIK DAN CASEIN
HYDROLISATE PADA PERTUMBUHAN IN VITRO EMBRIO ANGGREK
Spathoglottis plicata Blume</title><creator>, TRI SARTIKONINGSIH,S.P.</creator><creator>, Dr. Endang Semiarti, M.S. M.Sc.</creator><subject>ETD</subject><description>Orchids are widely used by society as ornament plant and cutting flowers.
The technique of in vitro culture using a combination of anorganic fertilizer and
organic complex casein hydrolisate as culture medium were develop for
micropropagation. The aims of this research are to know whether anorganic
fertilizer medium can be used as that of NP medium, to decide the concentration
of casein hydrolisate should be added to anorganic fertilizer medium to improve
the growth in vitro culture of Spathoglottis plicata Blume embryos.
In this research, combination between (0, 1, 2, 3) gr/l anorganic fertilizer
with a series (0, 25, 50, 75, 100) mg/l concentration of casein hydrolisate were
used as in vitro culture medium. The experiments were repeated 3 times.
Morphological characters were determined on the length of embryo(s) and
number of leaves, also colour of developping embryo (protocorms). Quantitative
data were analyzed using analysis of variance (ANAVA) and 1 % DMRT
(Duncan�s Multiple Range Test).
The Result showed that 1 gr/l of Gw 32-10-10 + 75 mg/l casein
hydrolisate and 1 gr/l of Gd 20-15-15 + 50 mg/l casein hydrolisate anorganic
fertilizer can be used as culture medium of S. plicata orchid with 57.6 % and
61.5 % growth frequency, respectively.</description><publisher>[Yogyakarta] : Universitas Gadjah Mada</publisher><date>2011</date><type>Thesis:Thesis</type><type>PeerReview:NonPeerReviewed</type><identifier> , TRI SARTIKONINGSIH,S.P. and , Dr. Endang Semiarti, M.S. M.Sc. (2011) PENGARUH KOMBINASI PUPUK ANORGANIK DAN CASEIN HYDROLISATE PADA PERTUMBUHAN IN VITRO EMBRIO ANGGREK Spathoglottis plicata Blume. UNSPECIFIED thesis, UNSPECIFIED. </identifier><relation>http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=53020</relation><recordID>90695</recordID></dc>
|
| format |
Thesis:Thesis Thesis PeerReview:NonPeerReviewed PeerReview |
| author |
, TRI SARTIKONINGSIH,S.P. , Dr. Endang Semiarti, M.S. M.Sc. |
| title |
PENGARUH KOMBINASI PUPUK ANORGANIK DAN CASEIN
HYDROLISATE PADA PERTUMBUHAN IN VITRO EMBRIO ANGGREK
Spathoglottis plicata Blume |
| publisher |
[Yogyakarta] : Universitas Gadjah Mada |
| publishDate |
2011 |
| topic |
ETD |
| url |
https://repository.ugm.ac.id/90695/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=53020 |
| contents |
Orchids are widely used by society as ornament plant and cutting flowers.
The technique of in vitro culture using a combination of anorganic fertilizer and
organic complex casein hydrolisate as culture medium were develop for
micropropagation. The aims of this research are to know whether anorganic
fertilizer medium can be used as that of NP medium, to decide the concentration
of casein hydrolisate should be added to anorganic fertilizer medium to improve
the growth in vitro culture of Spathoglottis plicata Blume embryos.
In this research, combination between (0, 1, 2, 3) gr/l anorganic fertilizer
with a series (0, 25, 50, 75, 100) mg/l concentration of casein hydrolisate were
used as in vitro culture medium. The experiments were repeated 3 times.
Morphological characters were determined on the length of embryo(s) and
number of leaves, also colour of developping embryo (protocorms). Quantitative
data were analyzed using analysis of variance (ANAVA) and 1 % DMRT
(Duncan�s Multiple Range Test).
The Result showed that 1 gr/l of Gw 32-10-10 + 75 mg/l casein
hydrolisate and 1 gr/l of Gd 20-15-15 + 50 mg/l casein hydrolisate anorganic
fertilizer can be used as culture medium of S. plicata orchid with 57.6 % and
61.5 % growth frequency, respectively. |
| id |
IOS2744.90695 |
| institution |
Universitas Gadjah Mada |
| institution_id |
19 |
| institution_type |
library:university library |
| library |
Perpustakaan Pusat Universitas Gadjah Mada |
| library_id |
488 |
| collection |
UGM Repository |
| repository_id |
2744 |
| subject_area |
Karya Umum |
| city |
SLEMAN |
| province |
DAERAH ISTIMEWA YOGYAKARTA |
| repoId |
IOS2744 |
| first_indexed |
2016-09-14T18:27:01Z |
| last_indexed |
2016-09-22T21:40:55Z |
| recordtype |
dc |
| _version_ |
1765816212719140864 |
| score |
17.538404 |