FORMULASI NANOPARTIKEL KITOSAN RANTAI PENDEK DAN KITOSAN RANTAI PENDEK-TPP SEBAGAI SISTEM PENGHANTARAN GEN NON VIRAL YANG DITRANSFEKSI PADA SEL KANKER PAYUDARA T47D
| Main Authors: | , LINA WINARTI, S.FARM, APT, , Dr.rer.nat.Ronny Martien, MSI. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2011
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/90193/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52585 |
Daftar Isi:
- Recently numerous prototype DNA-based biopharmaceuticals can be used to control disease progression by induction and inhibiting the overexpression of the genes. Since there are poor cellular uptake and rapid in vivo degradation of DNA-based therapeutics the use of delivery systems to facilitate cellular internalization and to preserve their activity is important. Cationic polymers commonly be used as carriers of a gene because of easily to form complexes and higher stability compared to lipoplexs. Chitosan a cationic polymer most widely be used in gene delivery systems because of low toxicity, and biocompatibility. The purpose of this study was to formulate nanoparticles of short chain chitosanpEGFP- C1 by complex coaservation method. Stability test of the formulas was performed by incubating the nanoparticles complex with DNase I and artificial intestinal fluid. Cytotoxicity and transfection studies were evaluated against T47D cell line. The diameter of Chitosan-pEGFP-C1 nanoparticles was on the range of 56 nm � 282.8 nm. The zeta potential was determined to be +14.03 - +16.6 mV. Stability studies showed that chitosan-pEGFP-C1 nanoparticles was stable, not degradated by DNase I for up to 1 hour and artificial intestinal fluid for 4 hour. Cytotoxic assay of ChitosanpEGFP- C1 nanoparticles (pH 4.0) showed that the viability of cell was > 90% for all formulas. EGFP-C1 and EFneo-GFP plasmid delivered by chitosan nanoparticles can be expressed in T47D cell culture. According to these results chitosan nanoparticles and chitosan-TPP were potentially to be used as a non-viral vector system delivery for gene therapy.