PENGARUH MARMIN DARI Aegle marmelos Corr. TERHADAP KONTRAKSI OTOT POLOS TRAKEA MARMUT TERISOLASI YANG DIINDUKSI SECARA IMUNOLOGIS DAN NONIMUNOLOGIS
| Main Authors: | , JOKO TRI WIBOWO, , Dr. Agung Endro Nugroho, M.Si., Apt. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2011
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/90123/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52497 |
Daftar Isi:
- Marmin is a coumarin derivative compound that was isolated from the stem bark and root of plants Maja (Aegle marmelos Corr.). Marmin reported to have spasmolytic effect on the guinea pigs ileum contracted by either histamine or acetylcholine. Marmin also reported able to inhibit histamine release from RBL-2H3 cell cultures. Based on the facts, marmin have the opportunity to be developed as a drug for allergy. This research was conducted to determine the effect of marmin on guinea pig tracheal smooth muscle contraction induced by both endogenous or exogenous histamine, and also the effects of marmin in smooth muscle relaxation. Ovalbumin (3 Âμg/mL) was used to induce guinea pig tracheal smooth muscle contraction immunologically, whereas compound 48/80 (2.5 to 750 Âμg/mL) were used to induce contractions non-immunologically. In this study, the influence of marmin was tested in guinea pig tracheal smooth muscle contraction that induced by series concentration of histamine (10 -8 -10 -4 M). In addition, test relaxation marmin (5-100 Î1⁄4M) on isolated guinea pig trachea was also conducted. The results showed that marmin 100 Î1⁄4M able to inhibit contractions of isolated guinea pig tracheal smooth muscle induced non-immunologically by compound 48/80. The average of inhibitory percentage of marmin on tracheal contraction induced by compound 48/80 750 Âμg/mL was 90.95±2.82%. Marmin (10 and 100 Î1⁄4M) was also able to inhibit guinea pig tracheal smooth muscle contraction induced immunologically by ovalbumin. The average of inhibitory percentage of marmin 10 Î1⁄4M and 100 Î1⁄4M marmin were 25.86±7.57% and 43.49±9.24%, respectively. Marmin 100 Î1⁄4M was also able to shift pD2 histamine from 6.02±0.05 to 4.94±0.07. In addition, marmin was able to relax guinea pig tracheal smooth muscle and have a pD2 relaxation of marmin of 4.42 ± 0.02.