EKSPRESI TRANSFORMING GROWTH FACTOR Î2-1 (TGFÎ2-1) PADA LUKA OPERASI TIKUS PUTIH HIPERGLIKEMIK PASCA INDUKSI STREPTOZOTOCIN DAN DIET LEMAK TINGGI
| Main Authors: | , drh. Tisna Tyasasmaya, , Dr. drh. Dhirgo Adji, M.P. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2011
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/39134/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50290 |
Daftar Isi:
- Surgery in a diabetic patient needs to have a serious concern because of its different phase of wound healing. It is also important to do a nutritional evaluation to the patients to get a better post-surgical wound healing. The objective of this research was to observe the expression of TGFÎ2-1 in wound healing of the rats injected with STZ. Fourty female Sprague Dawley rats, 2 months of age, 200 grams of body weight were used in this research. Rats were adapted for a week, fed in a standard diet and water ad libitum. Rats were then divided into 2 groups, 20 for each. Group I was fed in standard diet containing 5% fat and Group II was fed in high fat diet containing 30% fat. After 30 days on diet treatment, each group was divided into 4 subgroup. Group 1.1, 1.2, 1.3 and 1.4 were fed in a standard diet which is group 1.1 and 1.2 were not injected by STZ but group 1.2 was treated with cutaneous surgery. Group 1.3 and 1.4 were injected by STZ but group 1.4 was treated also with surgery. Group 2.1, 2.2, 2.3 and 2.4 were fed on high fat diet, which is group 2.1 and 2.2 were not injected by STZ but group 2.2 was treated with surgery whereas group 2.3 and 2.4 were fed on a high diet and injected by STZ but group 2.4 was also treated with surgery. The dose of STZ for each rat was 50 mg/kg body weight (BW) intraperitoneally. Five days after STZ injection, group 1.2, 1.4, 2.2 and 2.4 were incised aseptically at the dorsal area for about 3 cm in length. The wound areas were sutured with simple interrupted method using silk material and smeared with iodin liquid. Five days post-surgical, ratâ��s blood was collected from orbital vein and kept into uncoagulated tube for glucose and triglyceride measurement. After that, rats were killed to collect the skin tissue for histopathologic analysis using HE staining and also immunohistochemistry to analyze the expression of TGFÎ2-1 using Streptavidin-Biotin method. All the collected datas such as glucose and triglyceride concentration were analyzed statistically using factorial method, while the histopathologic and immunohistochemistry specimen were analyzed descriptively based on the alteration of the tissues and appearance of dark brown color on the skin tissues. Based on statistical analysis of the data showed that glucose concentration was affected by STZ (P<0,05), but not by the diet or surgery (P>0,05), while triglyceride concentration was affected by the three factors: diet, STZ and surgery (P<0,05). Based on histopathologic analysis of ratâ��s skin showed that there were an increase of inflammation cells at the wound area from the group of rats fed by high fat diet, stz injection and surgery whereas the immunohistochemistry showed a positive result in the same group. Based on those analysis, it is concluded that the expression of TGFÎ2-1 appeared on ratâ��s skin was a marker in inflammation phase of wound healing, but it is not clear yet whether the role of TGFÎ2-1 as a promoter or inhibitor because the skin was collected three days post-surgery which is considered as an inflammation phase of wound healing.