Application of Random Amplified Polimorphic DNA (RAPD) Markers for Detection of Powdery Mildew Resistence Gene In Diverse Melons
| Main Author: | Daryono, Budi Setiadi |
|---|---|
| Format: | Proceeding PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2009
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/37677/1/Proceeding%20ICBS%202009.pdf https://repository.ugm.ac.id/37677/ |
Daftar Isi:
- pBUC114 is a random amplified polymorphic DNA (RAPD) primer amplified a DNA band in 1060 bp linked to powdery mildew resistence gene (Pm-W) in PMAR5, a powdery mildew resistent melon cultivar. In this study, the RAPD primer was used for Polymerase Chain Reaction (PCR)-RAPD to detect Pm-W gene in 16 melon cultivars. Results of PCR-RAPD showed that among 16 melon cultivars, 4 cultivars (WMR29, Harukei, Action 434 and Nigeashi) did not obtained a DNA band in 1060 bp linked to Pm-W gene and they are suspectible. Whereas, 12 cultivars (PMAR5, PMR45, PMR5, Action 434, PI 414723, PI 124112, PI 124111, PI 371795, Sunrise, MR1, Nigeashi-2, PI 161375 and Kohimeuri) obtaine a DNA band in 1060 bp linked to Pm-W gene and they are resistant. The result of this study revealed that RAPD markers can be used as a tool for rapid screening of resistent melons against powdery mildew. Key words: melon, Pm-W, powdery mildew, RAPD