Selection of Phalaenopsis amabilis (L.) Blume Orchid Resistance to Hygromycin
| Main Authors: | Mercuriani, Ixora, Purwantoro, Aziz, Moeljopawiro, Sukarti, Jang, Seonghoe , Semiarti, Endang |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/35190/1/IJB_2013-Mercuriani_et_al.pdf https://repository.ugm.ac.id/35190/ http://ijbiotech.ugm.ac.id |
Daftar Isi:
- Examination of Phalaenopsis amabilis orchid resistance to hygromycin antibiotic is an important step to do prior to Agrobacterium-mediated genetic transformation in this orchids using Hygromycin phosphotransferase (HPT) gene as a selection marker in the T-DNA that harboring a desired gene to be transfered. We exposed the plant on hygromycin containing medium. The experiment was conducted using 6 weeks old P. amabilis protocorms. These protocorms were subcultured onto NP medium supplemented with various concentration of Hygromycin (0, 5, 10, 20, 1nd 40 mg.L-1). The number of survival protocorms were examined every week for 4 weeks after subcultured (WAS). The resistancy of hygromycin was calculated as ratio of death protocorms per total protocorms). The result showed that 10 mg.L-1 hygromycin with 1 weeks of application caused death close to LD 50. This data indicate that P. amabilis resistance to hygromycin treatment on the appropriate concentration 10 mg.L-1, and this concentration can be used for other purposes in orchid system