Ekspresi Bone Morphogenetic Protein-2 untuk Mengukur Efektivitas Biomaterial Freeze Dried Bovine Bone Xenograft (FDBBX) sebagai Bahan Penyambung Tulang = Expression of Bone Morphogenetic Protein-2 for Measuring Effective
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/29052/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12115 |
Daftar Isi:
- Osteogenic activity in normal fracture healing will start early at around 1 week after the repair, but not necessarily occur in fracture healing with the aid of grafts. Demineralized freeze dried bovine bone xenograft (DFDBBX) is a bone graft material made from cows that are widely produced recently to assist the process of bone grafting in humans, but has not been used on animals. It does not know how it works in helping to repair the bone. Twenty male SD rats weighing 150g, were divided into 2 groups of 10rats each. Group I was the control group, the rats were fractured and reconnected immediately using intramedullar pin fixation, Group II was a group of rats that were fractured and reconnected immediately using pin fixation intramedular plus graft material of freeze dried bovine bone xenograft. Fracture of the left femur was made in diaphysis part of bone.Evaluation of joints performed regularly at 1 week, 2 weeks, 4 weeks, 6 weeks and 8 weeks post surgery respectively by selecting at random, each group of 2 rats. Rats at each observation were collected two head I group, rats were then killed, the bone parts to be joined was taken and stored in 10% of formalin for histopathologic examination. Some pieces of the bones were used for the analysis of bone morphogeneticprotein 2 (BMP-2 ) by immunohistochemical method. The results were then analyzed descriptively to see the changes directly on the X-rays and tissue. Overall results of this study showed that the graft material ofDFDBBX were osteocondusive rather than osteoinductive properties. Nevertheless, the biomaterial is useful for orthopedic purposes primarily of handling cases with partial fracture of bone tissue which is lost. Due to the nature osteocondusive ofDFDBBX will make cells to grow and to unify the separa~ebone fragments.