Sensitivitas Probe Sag 1 dan Bag 1 untuk Deteksi Toxoplasma gondii pada Ayam Buras
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/29043/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12106 |
Daftar Isi:
- This study was aimed to determine the sensitivity of Sag1 and Bagl Probe to detect Toxoplasmagondii iil free-rearing chicken using dot blot hybridization method. Thirty serologically free-rearing chicken toxoplasmosis DNA were used as samples in this study. Sagl and Bagl probes were labeled by non radioactive Dig-ll-dUTP. The success of detection was based on the establishment of colored dot on the nylon membrane after detected with antibody-antiDig. The Sensitivity test of Sagl and Bagl probes in detection were conducted by making serial dilutions of the dot blot hybridization positive free-rearing chicken DNA. The results showed that 19 positive samples detected by Sagl and Bagl probe by dot blot hybridization method. The sensitivity of5.87 pg I JlI Bagl probe to detect free-range chicken DNA was 0.23 n,gI Jll, and sensitivity of 6.72 pg I JlISag1Probe was 0.45 ng I Jll.From the resuls above it can be concluded that the Bag 1 probe was more sensitive thanthat ofthe Sag1probe to detect Toxoplasmagondii of free-range chickenDNA.