Aktivitas antihiperurisemia ekstrak air jinten hitam (Coleus ambonicus Lour) pada mencit jantan galur balb-c dan standardisasinya = Antihyperurisemia activity of water extract of black seed (Coleus ambonicus Lour) in ..
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2011
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/28911/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11974 |
Daftar Isi:
- Telah dllakukan pengujian aktivitas antihiperurisemia secara in vivo dan standardisasl ekstrak air biji jinten hitam (Coleus ambonicus Lour). Ekstrak kering biji jinten hitam, lalu dilakukan pengujian antihiperurisemia secara in vivo terhadap mencit putih jantan' galur Balb-C yang diinduksi dengan kalium oksonat dosis 250 mg/kgBB. Hasil pengujian antihiperurisemia menunjukkan bahwa ekstrak tunggal biji jinten hitam dosis 200 mg/kgBB memiliki efek menurunkan kadar asam urat yang cukup signifikan. Hasil. Penelitian menunjukkan bahwa kadar asam urat dalam serum dalam kontrol negatif, positif, dan perlakuna, masing-masing sebesar 3,100 :i: 0,346 mg/dL, 0,200 ± 0,100 mg/dL dan 1,200 ± 0,561 mg/dL. Standardisasi meliputi parameter non spesifik dan spesifik. Hasil menunjukkan parameter spesifik, kadar fenolat total dalam ekstrak biji jinten hitam sebesar 0,664 ± 0,035 % dan kadar total flavonoid sebesar 0,400 ± 0,018 %. Hasil penetapan parameter non spesifik menunjukkan bahwa susut pengeringan, kadar air, kadar abu, kadar abu tidak larut asam, cemaran logam berat Pb dan Cd, dan cemaran aflatoksin berturut turut adalah 14,93-14,99%, 6,09-7,89%, 7,12-7,49%, 5,20-6,66%, 6,405 ± 0,211 ppm, 0,0096 ± 0, tidak terdeteksi. Effect of water extract of black seed on uric acid metabolism in hyperuricemia mice models were studied. The influence of water extract of black seed on uric acid biosynthesis was examined using mice treated by 250 mg/ body weight potassium oxonate intra peritoneal. The result showed that water extract of black seed (200 mg/kg body weight, per oral) have a significant effect in reduction in plasma uric acid levels in comparison to negative control, but less active than positive control (allopurinol). The levels of uric acid in mice plasma for negative control,blackseed extract and positivecontrolwere 3.100 ± 0.346 mg/dL, 0.200 ± 0.100 mg/dL dan 1.200 ± 0.561 mg/dL, respectively. The standardization performed by determined specific and non specific parameters based on National Bureau of Food and Drug, Republic of Indonesia. Specific parameters of water extract of black seed are phenolic content, and flavonoid content are 0.664 ± 0.035 % and 0.400 ±0.018 %, respectively. Non specific parameters of water extract of black seed are loss of weight, water content, ash content, ash insoluble acid content, heavy metals contamination (Pb and Cd), and aflatoxin residue are 14.93-14.99%, 6.09-7.89%, 7.12-7.49%, 5.20- 6.66%, 6.405± 0.211 ppm, 0.0096± 0, not detected, respectively.