SYNTHESIS, IDENTIFICATIONAND INVITRO ANTITUMOURPRESCREENING TEST OF TRIPHENYLTINBENZOATETOWARDS A HUMAN CERVICAL CARCINOMACELL LINE, HeLa
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2008
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/27937/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11000 |
Daftar Isi:
- ABSTRACT In this study, triphenyltin benzoate was synthesized first, and followed by antitumor prescreening test of the compound towards a human cervical carcinoma cell line, HeLa. Three reaction steps were employed to obtain the compound needed, i.e. 1) synthesizing of tetraphenyltin compound via insitu phenilmagnesiumbromide Grignard reaction to tin(lV)chloride, 2) synthesizing triphenyltin chloride via redistribution reaction of tetraphenyltin to tin(lV) chloride without any solvent, the reaction completed depends on the temperature, in this case a good results was achieved at temperature 220°C for 6 h, 3) finally, triphenyltin benzoate was produced through a methathetical reaction of triphenyltin chloride to an excess of sodium benzoate in ethanol. In vitro prescreening antitumour activity of the compound towards a human cervical tumour cell line, HeLa was ca"ied out following an enzyme linked immunosorbent assays (ELISA). By this method, the test ended with good promising results. This indicates by the ICsoof 170 nM which is compared wel/ to cisplatinum with 1Cso 950 nM. Keywords: redistribution reaction, methathetical reaction, eel/line, in vitro, antitumour.