Expression of DMRT1 in Quail Embryos (Coturnix coturnix japonica)
| Main Authors: | Kusumawati, Asmarani, Istiyawati, Ninik, Aldora, Alice, Prihatno, Surya Agus, Gustari, Sri, Budiyanto, Agung |
|---|---|
| Format: | Proceeding PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/278235/1/2%20FULL%20DOKUMEN%20Expression%20of%20DMRT1.pdf https://repository.ugm.ac.id/278235/ |
Daftar Isi:
- The sex differentiation in the quail is a complex process of formation and involves many genes. One of the genes suspected of having a major influence on genital formation is DMRT1. This study aimed to see the level of DMRT1 gene expression in the quail embryo. The sample of the study was the gonadal tissue of quail embryos obtained from the fertile quail eggs. Eggs are incubated and taken daily to be isolated from the gonadal genetic material. Genetic material in the form of DNA was used for molecular sexing using CHDW gene. RNA was used to measure the expression of DMRT1 gene in quail embryo. Measurement of gene expression on days 1-8 was confirmed by nanodrop spectrophotometer. The results show that DMRT1 gene expression has differences in male and female embryos. DMRT1 gene expression in male embryos has higher intensity than females. DMRT1 gene is also seen to have maximum expression in male embryo's aged 3-5 days. This DMRT1 gene may have an important role in the process of genital formation and differentiation of quail embryos, especially in males