The Allele and Genotype Distribution in SNP G. 408 C>G of FABP4 Gene in Kebumen Ongole Grade Cattle
| Main Authors: | Fathoni, Akhmad, Maharani, Dyah, Sumadi, Sumadi, Hartatik, Tety |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/276320/2/ISAG%20%20-Fathoni%202019.pdf https://repository.ugm.ac.id/276320/ |
Daftar Isi:
- The FABP4 gene is a gene played a role in the accumulation of fatty acids on the cell surface and affected animals growth. To evaluate the polymorphism of FABP4 gene in Kebumen Ongole Grade Cattle, we genotyped a single nucleotide polymorphism (SNP) g. 408 C>G. One hundred blood samples were isolated and used for Polymerase Chain Reaction (PCR). The PCR product (590 bp) was digested by Hpy188I restriction enzyme for genotyping using PCR-RFLP method. The result revealed two alleles (C and G) and two genotypes (CC and CG). The frequency of C allele (0.97) revealed higher than G allele (0.03). The homozygot CC genotype (0.94) frequency was higher than heterozygot CG genotype (0.06). The results of Chi-square statistic showed the population were in Hardy-Weinberg equilibrium. As a conclusion, the SNP g. 408 C>G of FABP4 gene may be suggested to determine the genetic diversity of Kebumen Ongole Grade cattle.