Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats
| Main Authors: | Latifah, Latifah, Kustantinah, Kustantinah, Maharani, Dyah, Hartatik, Tety |
|---|---|
| Format: | Proceeding PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/276055/1/ISAG%20Latifah%202019.pdf https://repository.ugm.ac.id/276055/ |
Daftar Isi:
- Melanocortin-4 receptor (MC4R), a prototypical G-protein coupled receptor (GPCR) that is highly expressed in the hypothalamus plays an important role in regulating feed intake and energy balance. The aim of this study was to identify the association between single nucleotide polymorphism (SNP) in MC4R gene with dry matter and crude protein intake in post-weaned Bligon goats. The dry matter and crude protein intake were estimated by in vivo method. Genomic DNA from Seventeen Bligon goats was extracted for genotyping analysis using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). One SNP (g.1079C/T) was identified by PCR-RFLP with Kpn1 restriction enzyme. The results revealed 2 genotypes (CC and CT), with frequencies of 0.71 and 0.29, respectively. Statistical analysis showed no significant association between MC4R genotype and dry matter and crude protein intake. In conclusion, SNP g.1079C/T in the MC4R gene is only useful for animal genotyping, but it cannot be used as a marker-assisted selection on dry matter and crude protein intake in Bligon goats.