Polymorphism study of BMP15 gene in Indonesian Goats
| Main Authors: | Maharani, Dyah, Elieser, Simon, Budisatria, I Gede Suparta, Batubara, Aron, Sari, Apriliana, Hariyono, Dwi Nur Happy |
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| Format: | Proceeding PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2020
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/276041/1/ISTAP%20BMP15.pdf https://repository.ugm.ac.id/276041/ |
Daftar Isi:
- Bone Moprogenetic Protein 15 (BMP15) is the family of Transforming Growth Factor β (TGFβ) superfamily which essential for early ovarian folliculogenesis. The aim of this study was to detect the genetic variation within BMP15 gene in four Indonesian goat breeds. A total of 27 blood samples of Gembrong, Kosta, Samosir, and Kacang goats reared in Indonesia Goat Research Center Sei Putih, North Sumatera were collected. Sequence alignment using of 4 samples represent each breed has revealed one synonymous mutation in position g.735A>G (position number refer to GenBank JQ320890), which did not induce the change of lysine in position 135. Genotyping based on SNP g.735A>G was accomplished using BbsI restriction enzyme with PCR-RFLP method. All three genotype (AA, GG, and AG) showed in Gembrong, while in Samosir and Kacang goat the GG genotype was absent. Interestingly, Kosta goat only has AA genotype. The A allele (83%) was higher than G allele (17%), followed with AA (70%, n=19), AG (26%, n=7), and GG (4%, n=1) genotypes in all sample breeds. The hardyweinberg equilibrium analysis resulted the sample population was not deviated (X2<5.59). It can be suggested the SNP g.735A>G might be used for further study in association the gene with reproductive traits in goat.