In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves

Main Authors: Mirajuddin, Mirajuddin, Kustono, Kustono, Ismaya, Ismaya, Budiyanto, Agung
Format: Article PeerReviewed application/pdf
Bahasa: eng
Terbitan: Fakultas Peternakan Universitas Gadjah Mada , 2010
Subjects:
Online Access: https://repository.ugm.ac.id/273136/1/1.%205%20th%20ISTAP%20International%20Seminar%20On%20Tropical%20Animal%20Production%20Proceedings%20Part%202.pdf
https://repository.ugm.ac.id/273136/
ctrlnum 273136
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/273136/</relation><title>In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves</title><creator>Mirajuddin, Mirajuddin</creator><creator>Kustono, Kustono</creator><creator>Ismaya, Ismaya</creator><creator>Budiyanto, Agung</creator><subject>Veterinary Sciences</subject><description>A research on in vitro fresh sperm preparation for maintaining sperm viability at storage temperature of l4 derajat C using tannin supplementation of lamtoro leaves (Leucaena leucocephala)had been carried out in both Physiology and Animal Reproduction Laboratory, Faculty of Animal Science, Universitas Gadjah Mada for fresh sperm preparation and sperm quality evaluation, during March to July of 2009.The experiment was designed using the Complete Random Design consisted of 5 treatments and 5 replications. Variables measured were viability, motility and morphology of the treated fresh sperm stored for 14 days at temperature of l4oC. Based on the analysis of variance, the treatments had significant effect (P&lt;0.05) on viability, motility and morphology of the sperm. In&#xD; terms of viability, Pl(64.07%+-0.45) it was higher than control (P0=26.71%+-0.54), but it was not different for P2(57.44%+-0.52), P3(49.49%+-0.71), and P4 (47.83%+-0.61). The progress and very progress motility of spermatozoa were only found in control, and not in others. Non-progressive spermatozoa proportion of Pl, P2, P3 and P4,was found respectively. They were significantly higher than control.Forthe morphology, P4 had lower normal spermatozoa percentage than P0, Pl, P2 and&#xD; P3, rvhereas among P0, P l, P2, and P3, there were not different (P&gt;0,05).</description><publisher>Fakultas Peternakan Universitas Gadjah Mada</publisher><date>2010</date><type>Journal:Article</type><type>PeerReview:PeerReviewed</type><type>File:application/pdf</type><language>eng</language><identifier>https://repository.ugm.ac.id/273136/1/1.%205%20th%20ISTAP%20International%20Seminar%20On%20Tropical%20Animal%20Production%20Proceedings%20Part%202.pdf</identifier><identifier> Mirajuddin, Mirajuddin and Kustono, Kustono and Ismaya, Ismaya and Budiyanto, Agung (2010) In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves. Proceedings Part 2 "Community Empowerment and Tropical Animal Industry. pp. 565-571. ISSN 978-979-1215-21-3 </identifier><recordID>273136</recordID></dc>
language eng
format Journal:Article
Journal
PeerReview:PeerReviewed
PeerReview
File:application/pdf
File
author Mirajuddin, Mirajuddin
Kustono, Kustono
Ismaya, Ismaya
Budiyanto, Agung
title In vitro fresh sperm preparation for maintaining sperm viability at Storage temperature of l4oC using tannin supplementation of lamtoro leaves
publisher Fakultas Peternakan Universitas Gadjah Mada
publishDate 2010
isbn 9789791215213
topic Veterinary Sciences
url https://repository.ugm.ac.id/273136/1/1.%205%20th%20ISTAP%20International%20Seminar%20On%20Tropical%20Animal%20Production%20Proceedings%20Part%202.pdf
https://repository.ugm.ac.id/273136/
contents A research on in vitro fresh sperm preparation for maintaining sperm viability at storage temperature of l4 derajat C using tannin supplementation of lamtoro leaves (Leucaena leucocephala)had been carried out in both Physiology and Animal Reproduction Laboratory, Faculty of Animal Science, Universitas Gadjah Mada for fresh sperm preparation and sperm quality evaluation, during March to July of 2009.The experiment was designed using the Complete Random Design consisted of 5 treatments and 5 replications. Variables measured were viability, motility and morphology of the treated fresh sperm stored for 14 days at temperature of l4oC. Based on the analysis of variance, the treatments had significant effect (P<0.05) on viability, motility and morphology of the sperm. In terms of viability, Pl(64.07%+-0.45) it was higher than control (P0=26.71%+-0.54), but it was not different for P2(57.44%+-0.52), P3(49.49%+-0.71), and P4 (47.83%+-0.61). The progress and very progress motility of spermatozoa were only found in control, and not in others. Non-progressive spermatozoa proportion of Pl, P2, P3 and P4,was found respectively. They were significantly higher than control.Forthe morphology, P4 had lower normal spermatozoa percentage than P0, Pl, P2 and P3, rvhereas among P0, P l, P2, and P3, there were not different (P>0,05).
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