Karakterisasi Fenotipe Isolat Staphylococcus aureus Dari Sampel Susu Sapi Perah Mastitis Subklinis = Phenotyping of Staphylococcus aureus ..
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2005
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/25584/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=8580 |
Daftar Isi:
- Staphylococcus aureus merupakan salah satu penyebab utama mastitis pada sapi perah yang menimbulkan kerugian ekonomi yang cukup besar akibat turunnya produksi susu. Penelitian ini bertujuan untuk isolasi dan karakterisasi S. aureus yang diisolasi dan susu sapi perah di Kaliurang, Bantul, Boyolali dan Baturaden Jawa Tengah. Karakterisasi S. aureus meliputi uji clumping factor, uji koagulase, produksi hemolisin, produksi pigmen dan uji kepekaan S. aureus terhadap beberapa antibiotika. Dalam penelitian ini berhasil diisolasi dan diidentifikasi sebanyak 32 isolat S. aureus. Semua isolat positif pada uji clumping factor dan koagulase. Alfa-hemolisis dapat diamati pada 1 isolat, 7 isolat mempunyai sifat a dan 13-hemolitik, 11 isolat 13- hemolitik dan 13 isolat bersifat non-hemolitik. Berdasar produksi pigmen, 8 isolat menghasilkan pigmen berwarna oranye, 10 isolat pigmen kuning dan 8 isolat menghasilkan pigmen putih. Hasil uji resistensi antibiotik terlihat bahwa 24 isolat (75%) sensitif dan 8 isolat (25%) resisten terhadap ampisilin. Uji terhadap eritromisin diketahui 4 isolat (12,5%) sensitif, 5 isolat (15,57%) intermediet dan 23 isolat (71,97%) bersifat resisten. Sebanyak 25 isolat (78,13%) diketahui bersifat intermediet dan 7 (21,87%) resisten terhadap gentamisin. Sebanyak 4 isolat S. aureus (712,5%) bersifat sensitif dan 28 isolat (87,5%) resisten terhadap oksasilin. Terhadap tetrasiklin memperlihatkan sifat intermediet pada 19 isolat (59,38%), 12 isolat (37,46%) bersifat resisten dan hanya 1 isolat (3,16%) bersifat sensitif. Kata kunci: Staphylococcus aureus, susu sapi perah, fenotipe Staphylococcus aureus is recognized as a major pathogen causing mastitis in dairy cows. The disease causes significant economic loss due to decreasing of milk production. The research was designed to isolate and characterize S. aureus from milk samples of dairy cows in Kaliurang, Bantul, Boyolali and Baturaden, Central Java. The characterization of S. aureus based on clumping factor and coagulation assay, production of hemolysins and pigmens, and antibiotic sensitivity tests. Thirty two isolates were identified as S. aureus. All 32 isolates were positive for clumping factors and coagulases. An a-hemolysis could be observed in 1 isolate, a, I3-hemolysis for 7 isolates, 13-hemolysis for 11 isolates, and non-hemolysis in 9 isolates. Among 32 isolates revealed that 8 isolates produced an orange pigment, 10 isolates were yellow pigmented and white pigmented for 14 isolates. The antibiotic resistance tests showed that 24 isolates (75%) were susceptible, and 8 isolates (25%) were resistant to ampicilin. Four isolates (12.5%) were susceptible, 5 isolates (15.57%) intermediate, and 23 isolates (71.97%) were resistant to erythromycin. Twenty four isolates (78.13%) were intermediate and 7 isolates (21.87%) were resistant to gentamicin. Four S. aureus isolates (12.5%) were susceptible and 28 isolates (87.5%) resistant to oxacillin. To tetracycline showed that 19 isolates (59.38%), were intermediate, 12 isolates (37.46%) resistant and only 1 isolate (3.16%) were susceptible. Key words: Staphylococcus aureus, milk dairy cow, phenotype