Kandungan Enzim Kholinesterase dan Icreatendi pada Petani Penebar Pestisida di Kabupaten Brebes*):Cholinesterase and crea7inine content on the farmer, working with
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 1996
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/24523/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=7500 |
Daftar Isi:
- Peningkatan agroindustri erat hubungannva dengan peningkatan pernakaian pestisida yang merupakan senyawa yang roksik untuk makluk hidup termasuk manusia. Pestisida yang banyak dipakai di indonesia adalah golongan penghambat enzim kholinesterase. Kadar enzim kholinesterase dapat dipakai sebagai salah satu petanda biologis untuk keracunan pestisida golongan penghambat enzim kholinesterase. Penelitian ini dicoba untuk inenganalisis kadar enzim kholinesterase pada petani penebar pestisida /insektisida. Daerah penelitian ini adalah Desa Tersana Kabupaten Brebes, merupakan desa yang banvak menggunakan pestisida. Penelitian ini dikerjakan dengan cara mengambil darah vena dari petani sebanyak 2 ml dicampur antikoagulan heparin. Data tentang, kondisi badan petani, lama dan seringnya meneharkan pestisida, teknik dan perlengkapan pelindung yang digunakan dapat menggambarkan besarnya interaksi pestisida kedalam badan petani pemakai, dikumpulkan dad petani yang diambil darahnva. Kadar enzim kholinesterase diukur dengan cara spektrofotometri menggunakan reagen "Merckotest7S-Butiril tiokholin iodida) sedangkan kreatinin plasma menggunakan reagen kreatinin test. Hasa pemeriksaan dari a0 responden dapat disimpulkan hahwa kadar kholinesterase bagi orang normal (standar)=2,575 petani dengan kadar (0-0,643) kLYl Keracunan Berat = (KB) sebanyak I orang = 2,5%, kadar (0,956-1.287) kU/1 Keracunan &clang (KS) sebanyak 5 orang= 12.5%, kadar (1,593-1,611) kali Keracunan Ringan = (KR) sebanyak 15 orang �37,5%, kadar (1,931-2,567) kU/1 Kondisi Normal = (N) sebanyak 19 arcing = -17,5%.Kadar kreatinin dari responden dipengaruhi oleh besarnya paparan pestisida. Kata kunci Kholinesterase. Pestisida, Petani, Kreatinin ABSTRACT Increasing of agroindustries product is closely related to increasing the pesticide usage. Pesticide is a toxic substance for living organism including human being. Pesticides group of organophosphat and carbamat that have ability to interaction with cholinesterase are commonly used in Indonesia. The total blood concentration of cholinesterase could be used as one of biological marker for toxicity of organophosphat and carbamat group. This research is aimed to identify the1concentration of cholinesterase eri.zynie in the farmer blood which working with pesticide/insecticide. The study was carried out in Tersana, grebes. The farmer in that village are using a lot of pesticides for their crops. The sampel was done by taking of 2 ml the veik blood from the farmer, and adding heparin as anticoagulant. The periode, frequently, and using of equipment during the farmer ~rack. are closely related to the condition of working farmer, that had been collected by quistioner/dialogue. Cholinesterase enzyme concentratiqp is analyzed spectrophotometrically using .1.1erckoiest reagent, and creatinine using creatinine test reagent. Result of this investigation could be summerized that cholinesterase consentration in normal people (standard) is 2,575 kl.1, the farmer which have the value of (0-0,643) kU/1 Hard Toxicity = (HT) is 1 person 2.5%. the value of (0,956-1,287) kU/I Midle Toxicity = (MT) are 5 persons = 12:5%, the value of ( 1.593-1.611) kUil Light Toxicity = (LT) are 15 persons = 37,5%, the value of (1,931-2,567) kUl Normal Conditon = (N) are 19 persons = 47,5% The creatinine concentration in each respondent was influenced by insecticide interactions. Key word : Cholinesterase, Pesticide, The farmer. C.'reatinine