Development of DNA recombinant vaccine for type 1 bovine viral diarrhea virus in vitro
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 1997
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/23454/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=6403 |
Daftar Isi:
- A DNA recombinant bovine viral diarrhea virus (BVDV) vaccine based on adenovirus vector has been developed for this purpose. The recombinant adenovirus type 5 (Ad5) contains a deletion of a portion of the El region. In the present study, the foreign genes for BVDV of type 1 those are the 53 Kd glycoprotein (gp53) and 53 Kd glycoprotein with signal peptide sequence (gp53s) were inserted into adenovirus genome vector, the Ad5 strain within the El region. In this case, the gp53 and gp53s of BVDV were expressed by the Ad5 vector in 293 cell line of human embryonic kidney, in which Ad5 replication is efficient. It was concluded that El-deleted Ad5 is suitable for expressing BVDV genes) and has the potential advantage of being used as a general approach to a DNA recombinant vaccination of BVDV. In addition, the researchs on the incidence of BVDV infection in Indonesian cattle using immunoperoxidase monolayer assay (IPMA) have recently been developed and performed in the Laboratory of Immunochemistry/Chemistry, Inter University Center for Biotechnolgy, Gadjah Mada University, Yogyakarta, Indonesia. The noncytopathic biotype of bovine viral diarrhea virus (NCP-BVDV) is isolated in Indonesian cattle and seems to pose significant health problem in livestock industry in Indonesia. In addressing the global needs for effective control of BVDV in Indonesia, additional experiment(s) in the use of Ad5 vector in the DNA recombinant vaccine production of NCP-BVDV of Indonesian (local) isolates are being developed. Keywords: Recombinant adenovirus type 5 � type 1 BVDV strain � gp53-gp53s � human embryoni