Application of the immunoperoxidase monolayer assay to characterize non-cytopathic bovine viral diarrhea virus of Indonesian isolates
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 1997
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/23392/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=6339 |
Daftar Isi:
- The approach of the immunoperoxidase monolayer assay (IPMA) was used to characterize 2 different fragments of non¬cytopathic bovine viral diarrhea virus (NCP-BVDV) genes of Indonesian strains, isolated in the Immunochemistry/Chemistni Laboratory at Inter University Center for Biotechnology, Gadiah Mada University, Yogyakarta, Indonesia. Each strain was tested for reactivity with a monoclonal antibody (MoAb) specific for two different regions within the gene coding for protein p80 and within the envelope glycoprotein (gp53) region. The results indicated that only one NCP-BVDV of Indonesian isolate, designated 650 strain which reacts with the p80 and all of the gp53 specific MoAbs. Whereas, others had a different pattern of reactivity with MoAbs tested. The variability of the NCP-BVDV reaction against MoAbs used in the present study may reflect in their antigenic variability. Keywords: NCP � BVDV � Indonesian isolate � p80 gene � gp53 gene